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Article type: Research Article
Authors: W.S. Perera, | M.T. Moss, | S.J. Urbaniak,
Affiliations: Academic Transfusion Medicine Unit, Department of Medicine and Therapeutics, University of Aberdeen, Aberdeen AB25 2ZD, Scotland, UK
Note: [] Academic Transfusion Medicine Unit, Regional Transfusion Centre, Foresterhill, Aberdeen AB25 2ZW, Scotland, UK. Tel.: +44 1224 685685; Fax: +44 1224 695351; E-mail: w.s.perera@abdn.ac.uk
Abstract: Our 11 anti-RhD's in conjunction with 37 previously published RhD antibodies, produced by hybridoma technology were analysed for germline gene usage and restriction in V_{\rm H} and V_{\rm L} pairings. The 17 V_{\rm H}germline genes used by the hybridoma anti-RhD IgG were derived from 4 VH families (VH1, VH2, VH3 and VH4). Eighteen kappa chains were restricted to only 5 germline genes from only 2 V\kappafamilies (V\kappa1 and \kappa3). However, the 13 lambda chains were not as restricted, using 10 V_{\lambda} germline genes from 4 families (V\lambda1, V\lambda2, V\lambda3 and V\lambda8). Fifty six unique Fab/phage anti-RhD were also analysed. In all cases the Fab/phage V_{\rm H} germline genes were derived from the VH3 family (41/41). The 29 kappa chains were restricted to 4 germline genes primarily from V\kappa1 (97%) germline genes from 5 families (V\lambda1, V\lambda2, V\lambda3, V\lambda4 and V\lambda7). The V_{\rm H} germline genes of the Fab/phage were restricted to 4 of the 17 used by the hybridoma anti-RhD IgG (DP46, DP49, DP50 and DP77). Ninety percent of the Fab/phage were restricted to 1 of the 5 V_{\kappa}germline genes used by the IgG (DPK9). However, the repertoire of the V_{\lambda}germline genes used in these two systems is different, with analysis showing greater diversity in V_{\lambda} gene usage with 8 unique germline genes used by 76% Fab/phage compared to 4 unique genes used by 46% hybriboma anti-RhD.
Keywords: Anti-RhD, germline gene, canonical structure, V[TeX:] _{\rm H} and V[TeX:] _{\rm L} pairings
Journal: Disease Markers, vol. 16, no. 1-2, pp. 15-19, 2000
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