Validation of a method for erythrocyte aggregation measurement using light back-scattering

Issue title: Red Cell Aggregation. Proceedings of the Second International Symposium. Paris, France, January 1988

Guest editors: J.F. Stoltz

Article type: Research Article

Authors: Pignon, B.^a | Muller, S.^b | Jolly, D.^a | Siadat, M.^b | Petitfrere, E.^a | Vessel, B.^c | Donner, M.^b | Potron, G.^a | Stoltz, J.F.^b

Affiliations: [a] Laboratoire d’Hématologie, Hôpital Robert-Debré, rue Alexis Carrel 51092, Reims, Cédex, France | [b] INSERM U 284, CO 10 Plateau de Brabois 54511, Vandoeuvre-Les-Nancy, Cédex, France | [c] Unité de Rééducation Fonctionnelle, Hôpital Sébastopol, 51092, Reims, Cédex, France

Note: [] Accepted by: 

Abstract: A biological evaluation of the erythroaggregameter commercialized by SEFAM (Nancy, France) is reported. The main characteristics of the apparatus are: Couette-type viscometer, laser light source (wavelength near infra-red), analysis of back-scattered light Several quantitative parameters are calculated: aggregation times, partial and total dissociation thresholds. Reproductibility and variance are satisfactory. The method is very dependent on the experimental conditions such as: delay between sampling to measurement, temperature, nature of anticoagulant. We confirm the importance of both hematocrit and fibrinogen. There is a sex related difference of aggregation parameters which is not dependent on hematocrit. At last, we have found that erythroaggregameter was able to detect RBC damages induced by either moderate heating or surface charge alterations.

Keywords: Hemorheology, erythrocyte aggregation, light back-scattering

DOI: 10.3233/CH-1989-9505

Journal: Clinical Hemorheology and Microcirculation, vol. 9, no. 5, pp. 739-749, 1989