Affiliations: Research Center of Occupational Medicine, Peking University Third Hospital, Beijing 100083, PR China | Hemorheology Center, Department of Biophysics, School of Basic Medical Sciences, Peking University, Beijing 100083, PR China | Peking University Modern Research Center for Traditional Chinese Medicine, Beijing 100083, PR China
Note: [] Correspondingt author: Jin-Yuan Zhao, Research Center of Occupational Medicine, Peking University Third Hospital, Beijing 100083, PR China. Tel.: +86 10 6201 7691 2651; Fax: +86 10 6201 7700; E-mail: zhaojinyuan@sina.com.
Abstract: The present study was to investigate the protective effects of salvianolic acids (SA) on deformability of red blood cells (RBCs) and its mechanism during the development of acute lung injury (ALI) induced by oleic acid (OA) in rabbits. 32 rabbits were randomized into four groups, normal control group, OA-treated group (0.15 ml/kg), SA-treated group and OA+SA treated group. The blood samples were collected at 0, 10, 30, 60, 90, 120 and 180 min after OA injection. The RBC deformation index, Orientation index and small deformation index were measured by ektacytometry. The concentration of malondialdehyde (MDA) in RBCs was detected by the assay kit. Meanwhile, the pulmonary pathological examination and the blood gas analysis were also performed. The results showed that the deformation index, orientation index and small deformation index decreased during the early phase of ALI, while the concentration of MDA in RBCs increased during the course. Pre-treatment with SA increased the deformability and orientability of RBC significantly and decreased the concentration of MDA in RBCs compared with OA group. Meanwhile, the hypoxia and pulmonary pathological damage were much improved. These results suggest that there were erythrocyte deformability changes in the early phase of ALI. SA has the protective effects on erythrocyte deformability during the development of ALI induced by OA, which might be due to its antioxidant effect. These results are valid in rabbits and in a model of ARDS, it would be interesting to see the effects of SA in patients.
