Influence of physico-chemical and pathological factors on the individual red cell transit time

Issue title: The Fifth European Conference on Clinical Hemorheology. Part I

Article type: Research Article

Authors: Franzini, E.^a | Driss, F.^b | Darcet, Ph.^b | Driss, Fr.^c | Daoud, F.^b | Thao Chan, M.^a

Affiliations: [a] Hoechst Laboratories, Hemorheology lab., 92080 Paris, La Defense, France | [b] Hopital ste Perrine, 75016 Paris, France | [c] Hopital Laennec, 75007 Paris, France

Note: [] Accepted by: Guest Editor J.C. Healy

Abstract: Erythrocyte Deformability (ED) was investigated by a Cell Transit-time Analyser (CTA) in three populations: elderly subjects, liver diseases patients and controls. Different conditions of measures were evaluated: anticoagulants (EDTA and heparin), buffers (PBS and tris), temperatures (25° C and 37° C), and different pressures. The CTA analysed the deformability of individual cell passing through 5 um pores of a polycarbonate filter (Oligopore) under a low hydrostatic pressure. This method showed that E.D. was lower in elderly and in liver diseases groups than in the control group. Several fractions of less deformable red cells, as regard to their transit-times through the filter, were detected by the CTA. By contrast, red cells from controls group didn’t present these subpopulations.

Keywords: Erythrocyte Deformability, CTA, transit time, subpopulations

DOI: 10.3233/CH-1988-83-428

Journal: Clinical Hemorheology and Microcirculation, vol. 8, no. 3-4, pp. 485-492, 1988