Effect of beta adrenergic agents on granulocyte deformability

Article type: Research Article

Authors: Starc, Thomas J. | Schmalzer, Emily A. | Chien, Shu

Affiliations: Departments of Physiology and Pediatrics, College of Physicians and Surgeons, Columbia University, New York, New York, USA

Note: [] Accepted by: Editor G.D.O. Lowe

Abstract: The effect of beta adrenergic agents on granulocyte deformability was investigated using the filtration technique. Three groups of granulocytes were studied: 1) cells treated with isoproterenol (10−6 to 10−3 M); 2) cells treated with propranolol (10−6 to 10−3 M); and 3) control cells. Granulocytes were pumped at a constant rate through polycarbonate filters with 5 µm diameter pores. The pressure generated as the cell suspension was pumped through the filter reflected the deformability of the cells. Isoproterenol in concentrations ranging between 10−6 to 10−3 M and propranolol in concentrations < 3.5 × 10−4 M did not change the filtration pressure of granulocytes. Propranolol in concentrations ≥ 3.5 × 10−4 M increased the filtration pressure. This decrease in deformability of granulocytes was associated with a loss of pseudopod formation and a decrease in percent viability. High doses of propranolol decrease granulocyte deformability.

Keywords: Granulocyte Deformability, Filtration, Propranolol, Isoproterenol

DOI: 10.3233/CH-1987-7506

Journal: Clinical Hemorheology and Microcirculation, vol. 7, no. 5, pp. 699-706, 1987