The use of high gradient magnetic separation for the preparation of leucocyte- and platelet-free red cell suspensions

Article type: Research Article

Authors: Pockley, A.G.^{a; *} | Davenport, P.^a | Paul, F.^b | Greaves, M.^a | Preston, F.E.^a

Affiliations: [a] Department of Haematology, Royal Hallamshire Hospital, Sheffield, England | [b] Department of Physics and Haematology, Southampton University, Southampton, England

Note: [*] Present Address: Department of Biological Sciences, Sheffield City Polytechnic, Pond Street, Sheffield S1 1WB

Note: [] Accepted by: Editor G. Lowe

Abstract: The preparation of a leucocyte- and platelet-free red cell suspension produced by the use of high gradient magnetic separation (HGMS) is described. 0.5ml of whole blood is required for the production of 1.6 × 109 washed red cells and removal of 98.4% of leucocytes and 99.7% of platelets is achieved within 15 minutes. The technique also achieves a high degree of reproducibility. The red cells isolated are shown to be representative of the original population and show no preferential selection. High gradient magnetic separation represents an efficient, effective, yet simple method for the separation of erythrocytes from whole blood.

Keywords: Rheology, Erythrocyte indices, Magnetic separation

DOI: 10.3233/CH-1986-6503

Journal: Clinical Hemorheology and Microcirculation, vol. 6, no. 5, pp. 373-379, 1986