Red cell deformability and membrane protein phosphorylation in peripheral arterial disease

Article type: Research Article

Authors: Morlé, L.^a | Alloisio, N.^a | Heynen, Y.G.^b | Martin, J.-P.^c | Pasquier, J.^c | Delaunay, J.^a

Affiliations: [a] Groupe de Recherches sur le Globule Rouge, Faculté de Médecine, Grange-Blanche, 69373 Lyon Cedex 08, France | [b] Service de Chirurgie Vasculaire, Hôpital Edouard Herriot, 69374 Lyon Cedex 08, France | [c] Clinique Médicale A, Hôpital Edouard Herriot, 69374 Lyon Cedex 08

Note: [] Accepted by: Editor J.F. Stoltz

Abstract: Nine non-diabetic patients with peripheral arterial disease were investigated as for (i) their red cell deformability, using a filterability measurement technique and (ii) the in vitro phosphorylation of their red cell membrane proteins, with special reference to cytoskeletal proteins. The deformability appeared significantly (p < 0.01) reduced in the patients. However, the phosphorylation of erythrocyte membrane proteins was strictly normal. Therefore, the molecular basis for decreased deformability must not reside in some acquired alteration of the cytoskeleton, in vitro phosphorylation being a sensitive probe of cytoskeletal protein organization.

Keywords: Erythrocyte deformability, Membrane protein phosphorylation, Peripheral arterial disease

DOI: 10.3233/CH-1983-3501

Journal: Clinical Hemorheology and Microcirculation, vol. 3, no. 5, pp. 433-441, 1983