The influence of calcium ions and ionophore A23187 on microrheological characteristics of erythrocytes by new model ektacytometry

Article type: Research Article

Authors: Chunyi, Wang | Yanjun, Zeng^; | Weibo, Ka

Affiliations: Biomechanics and Medical Information Institute of Beijing Polytechnic University, Beijing 100022, P.R. China | Biophysics Department of Medical School, Peking University, Beijing 100083, P.R. China

Note: [] Corresponding author: Prof. Yanjun Zeng, Biomedical Engineering Center, Beijing Polytechnic University, Beijing 100022, P.R. China. Tel: +86 10 67392172; E‐mail: yjzeng@bjpu.edu.cn.

Abstract: After rabbit's erythrocytes were treated with different concentrations of intercellular Ca2+ and ionophore A23187 respectively, the orientation index (DI)or and small deformation index (DI)d of rabbit erythrocytes were measured by new model ektacytometry. The results demonstrated that (DI)or and (DI)d decreased while the concentration of intercellular Ca2+ or ionophore A23187 increased. Suggesting that the deformability of erythrocytes was reduced after treatment with intercellular Ca2+ or ionophore A23187. Furthermore, the effect of ionophore A23187 on the microrheology of erythrocytes is more notable than that of intercellular Ca2+.

Keywords: New model ektacytometry, calcium ions, ionophore A23187, erythrocyte deformation

Journal: Clinical Hemorheology and Microcirculation, vol. 24, no. 1, pp. 19-23, 2001