Comparison of three optical methods to study erythrocyte aggregation

Article type: Research Article

Authors: Zhao, H.^; | Wang, X. | Stoltz, J.F.

Affiliations: Angiohématologie–Hémorhéologie, LEMTA, UMR CNRS 7563, Faculté de Médecine, 54500 Vandoeuvre‐lès‐Nancy, France | China–Japan Friendship Research Institute of Clinical Medical Sciences, 100029 Beijing, China

Abstract: The aim of this work was to evaluate three optical methods designed to determine erythrocyte aggregation: Erythroaggregometer (EA; Regulest, France), Laser‐assisted Optical Rotational Cell Analyzer (LORCA; Mechatronics, Netherlands) and Fully Automatic Erythrocyte Aggregometer (FAEA; Myrenne, GmbH, Germany). Blood samples were taken from fifty donors (26 males and 24 females). The aggregation of normal red blood cell (RBC) and RBCs suspended in three normo‐ and hyper‐aggregating suspending media was studied. The results revealed some significant correlations between parameters measured by these instruments, in particular, between the indexes of aggregation of EA and LORCA. Further, RBC aggregation of multiple myeloma patients was also studied and a hyper erythrocyte aggregation state was found by EA and LORCA.

Keywords: Erythrocyte aggregation, laser back‐scattering, multiple myeloma, shear stress

Journal: Clinical Hemorheology and Microcirculation, vol. 21, no. 3-4, pp. 297-302, 1999