Influences of high-voltage electrical burns on the pulmonary microcirculation in rabbits

Article type: Research Article

Authors: Zhou, H.M.^{a; 1} | Xu, S.J.^{b; 1} | Wang, L.^c | Shao, H.B.^a | Xie, B.^b | Feng, J.K.^a | Wang, C.J.^a | Bai, Y.Q.^a | Zhang, Q.F.^{a; *}

Affiliations: [a] Department of Burns and Plastic Surgery, The First Hospital of Hebei Medical University, Shijiazhuang, China | [b] Central Laboratory, The First Hospital of Hebei Medical University, Shijiazhuang, China | [c] Department of Obstetrics and Gynecology, Hebei General Hospital, Shijiazhuang, China

Correspondence: [*] Corresponding author: Q.F. Zhang, Department of Burns and Plastic Surgery, The First Hospital of Hebei Medical University, Shijiazhuang, China. Tel.: +86 311 8591 7010; Fax: +86 311 8591 7290; E-mail: zhangqfdoctor@163.com.

Note: [1] These authors contributed equally to this work.

Abstract: This study was performed to investigate the effects of high-voltage electrical burns (HEB) on the pulmonary microcirculation in rabbits. Total of 120 rabbits were randomly divided into control and HEB group using a random number table. HEB model was developed with a voltage regulator and experimental transformer. Laser Doppler perfusion imager was utilized to monitor and quantify the blood perfusion in pulmonary microcirculation. The microvascular morphologic changes of the lung were observed using light microscopy and transmission electron microscope (TEM). The lung wet/dry weight ratio and the PaO2 were determined. The values of blood perfusion in rabbit pulmonary microcirculation in the HEB group were decreased at 5 min, but increased at 1 h after burn (P <  0.01) and then decreased gradually. Light microscopy reveals microthrombus formation in pulmonary venules and bleeding in venous capillaries in HEB group. We found the number of microvilli in the capillary endothelial cells decreased, the rough endoplasmic reticulum expanded and severe degranulation occurred, the mitochondrial cristae fused or disappeared, and severe edema surrounded the capillary endothelial cells by TEM. The values of lung wet/dry weight ratio were higher and the PaO2 were lower than that of before burn group (P <  0.01). These results demonstrated that microcirculatory disorders play a major role in the development of progressive lung damage after high-voltage electrical burns.

Keywords: Burn, electric injuries, lung, microcirculation

DOI: 10.3233/CH-141921

Journal: Clinical Hemorheology and Microcirculation, vol. 62, no. 3, pp. 193-203, 2016