Effects of extracts prepared from modified porous poly(ether imide) microparticulate absorbers on cytotoxicity, macrophage differentiation and proinflammatory behavior of human monocytic (THP-1) cells
Issue title: Special Issue in Honour of the Editor-in-Chief Prof. Dr. Ing. Friedrich Jung on his 70th Birthday
Affiliations: [a] Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India
| [b] Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany
| [c] Institute of Chemistry, University of Potsdam, Potsdam, Germany
| [d] Institute for Molecular Cardiovascular Research (IMCAR), RWTH Aachen University, University Hospital, Germany
| [e] School of Cardiovascular Diseases (CARIM), University of Maastricht, Universiteitssingel, Maastricht, The Netherlands
Correspondence:
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Corresponding author: Dr. Sarada D. Tetali, Professor, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, Telangana State, India. Tel.: +91 40 23134512; Fax: +91 40 23010120; E-mails: stetali@uohyd.ac.in or saradakanakagiri@gmail.com.
Abstract: Remaining uremic toxins in the blood of chronic renal failure patients represent one central challenge in hemodialysis therapies. Highly porous poly(ether imide) (PEI) microparticles have been recently introduced as candidate absorber materials, which show a high absorption capacity for uremic toxins and allow hydrophilic surface modification suitable for minimization of serum protein absorption. In this work, the effects of extracts prepared from PEI microparticles modified by nucleophilic reaction with low molecular weight polyethylene imine (Pei) or potassium hydroxide (KOH), on human monocytic (THP-1) cells are studied. The obtained results suggested that the extracts of Pei and KOH modified PEI absorbers have no negative effect on THP-1 cell viability and do not initiate the critical differentiation towards macrophages. The extracts did not enhance transcript or protein levels of investigated proinflammatory markers in THP-1 cells, namely, TNFμ, MCP1, IL6 and IL8. Based on these findings such modified PEI microparticles should be qualified for further pre-clinical evaluation i.e. in an in vivo animal experiment.
