Affiliations: Key Laboratory for Microcirculation, Ministry of National Health of China, Institute of Microcirculation, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China
Correspondence:
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Corresponding author: Ruijuan Xiu, Key Laboratory for Microcirculation, Ministry of National Health of China, Institute of Microcirculation, Chinese Academy of Medical Sciences & Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China. Tel.: +86 10 65126407; Fax: +86 10 65251957; E-mails: xiurj@imc.pumc.edu.cn, xiurj@aliyun.com.
Abstract: Pericytes are contractile cells that wrap around the endothelial cells of capillaries throughout the body. They play an important role in regulating the blood brain barrier (BBB) and blood spinal cord barrier (BSCB). The differences between brain and spinal cord microvascular endothelial cells have been investigated. However, no report has elucidated the similarities and differences between brain microvasular pericytes (BMPs) and spinal cord microvascular pericytes (SCMPs) in vitro. The similarities were found between the two types of pericytes not only in the proliferation ability but also in the expression of toll like receptor 4. On the other hand, BMPs showed more than 2 fold in tubular length formation compared with SCMPs. The number of migratory SCMPs was larger than that of migratory BMPs. The expressions of connexin 43 and vascular endothelial growth factor (VEGF) in BMPs were increased compared with those in SCMPs, while SCMPs expressed more desmin and N-cadherin than BMPs. The abilities of tube-formation and migration between BMPs and SCMPs were markedly different, which might be mediated by VEGF, connexin 43, N-cadherin and desmin. These distinguishing features may reflect the more widespread differences between the BBB and BSCB which directly impact pathophysiological processes in various major diseases.
Keywords: Brain microvasular pericytes, Brain microvasular pericytes, blood brain barrier, blood spinal cord barrier, central nervous system
