Presentation of a variation of the chorioallantoic membrane set up as a potential model for individual therapy for squamous cell carcinoma of the oropharynx

Issue title: Selected papers of the 36th Conference of the German Society for Clinical Microcirculation and Hemorheology, 5–8. June, 2017, Greifswald, Germany

Guest editors: M. Jünger, A. Krüger-Genge and F. Jung

Article type: Research Article

Authors: Kauffmann, P.^a | Troeltzsch, M.^a | Cordesmeyer, R.^a | Heidekrueger, P.I.^b | Schliephake, H.^a | Canis, M.^c | Wolff, H.A.^d | Rave-Fraenk, M.^d | Stroebel, P.^e | Kehrer, A.^f | Prantl, L.^f | Aung, T.^{f; *}

Affiliations: [a] Department of Maxillofacial Surgery (Head: Prof. Dr. Dr. H. Schliephake), University of Göttingen, Göttingen, Germany | [b] Department of Plastic, Reconstructive, Hand, and Burn Surgery, StKM – Klinikum Bogenhausen, Academic Teaching Hospital, Technical University Munich, Munich, Germany | [c] Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center Göttingen, Göttingen, Germany | [d] University Medical Center Göttingen, Present Address: Department of Radiotherapy and Radiation Oncology, Radiologie München, Munich, Germany | [e] Institute of Pathology, University Medical Centre, Göttingen, Germany | [f] Department of Plastic, Hand, and Reconstructive Surgery, University Medical Center Regensburg, Regensburg, Germany

Correspondence: [*] Corresponding author: Dr. Thiha Aung, Center of Plastic and Reconstructive Surgery, University Medical Center Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany. Tel.: +49 0 941 944 6763; E-mail: aung.anbs@gmail.com.

Abstract: The chorioallantoic membrane of fertilized chicken eggs in an early phase of breeding presents an approved test situation for the growth and treatment of human cancer cells. These models work due to the inoculation of cells into the membrane that stays within the egg shell during the time of investigation. In this study a modification of this model is presented. Samples of native tumors, rather than cell lines, are transplanted into the membrane and the body of the egg is taken out of the shell and placed in a plastic bowl. These modifications lead to an enhanced accessibility to the chorioallantoic membrane and the surrounding vessels thus facilitating intra venous access and application of pharmaceuticals and a focused radiotherapy. With the current modifications the embryo was kept alive and additionally, the vascularized tumor environment was preserved.

DOI: 10.3233/CH-179226

Journal: Clinical Hemorheology and Microcirculation, vol. 67, no. 3-4, pp. 453-457, 2017