Issue title: Hemostasis
Article type: Research Article
Authors: Aufradet, Emeline | DeSouza, Geneviève | Bourgeaux, Vanessa | Bessaad, Amine | Campion, Yannick | Canet-Soulas, Emmanuelle | Pialoux, Vincent | Chirico, Erica N.; | Chevrier, Anne-Marie | Godfrin, Yann | Martin, Cyril
Affiliations: EA Center of Research and Innovation on Sports, University Claude Bernard Lyon 1, University of Lyon, Lyon, France | ERYTECH Pharma, Lyon, France | INSERM, Laboratory CarMeN, University Claude Bernard Lyon 1, University of Lyon, Lyon, France
Note: [] Corresponding author: Cyril Martin, Ph.D., Center of Research and Innovation on Sports (CRIS EA647), Université Claude Bernard Lyon 1, Université de Lyon, Campus La Doua, Bat Raphael Dubois, 27-29 bd du 11 novembre 1918, 69622 Villeurbanne Cedex, France. E-mail: cyril.martin@univ-lyon1.fr
Abstract: In sickle cell disease, the factors involved in vasoocclusive crisis (VOC) include the sickling of red blood cells (RBC), abnormal blood rheology, inflammation, vascular adhesion, oxidative stress, coagulation, and vascular tone modulation. The aim of this study was to further characterize the molecular response of some factors involved in VOC by inducing a hypoxia/reoxygenation stress in sickle SAD mice. Results show that a hypoxia/reoxygenation stress in SAD mice can induce: (i) a decrease in reticulocytes count, and mean corpuscular volume along with an increase in lactate dehydrogenase (p = 0.07) and sickled cell proportion; (ii) a significant increase in lung VCAM-1, ICAM-1, IL-1β, ET-1, eNOS, and TF mRNA associated with an increase in VCAM-1 expression on lung endothelium; (iii) a rise in cardiac oxidative stress with increased lipid oxidation and decreased anti-oxidant enzyme activities, and (iv) an increase in plasma TNF-α and IL-6 and a decrease in plasma ET-1. In SAD mice, hypoxia/reoxygenation stress induces hemolysis that, together with oxidative stress, inflammation, vascular adhesion, and coagulation, may induce vascular occlusion and consequently RBC sickling. The present results give the kinetics of VOC molecular markers in SAD mice which may aid in testing the efficiency of new therapeutic processes against VOC.
Keywords: Sickle cell anemia, transgenic mice, inflammation, oxidative stress, adhesion molecules
DOI: 10.3233/CH-131735
Journal: Clinical Hemorheology and Microcirculation, vol. 54, no. 3, pp. 297-312, 2013
