Influence of nanoporous poly(ether imide) particle extracts on human aortic endothelial cells (HAECs)

Issue title: Selected papers of the 18th European Conference for Clinical Hemorheology and Microcirculation (ESCHM), 5-8 June, 2016, Lisbon, Portugal

Article type: Research Article

Authors: Kumar, Reddi K.^a | Basu, Sayantani^b | Lemke, Horst-Dieter^d | Jankowski, Joachim^{e; f} | Kratz, Karl^b | Lendlein, Andreas^{b; c} | Tetali, Sarada D.^{a; *}

Affiliations: [a] Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India | [b] Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany | [c] Institute of Chemistry, University Potsdam, Potsdam, Germany | [d] eXcorLab GmbH, Industrie Center Obernburg, Obernburg, Germany | [e] Institute for Molecular Cardiovascular Research (IMCAR), RTWTH Aachen University, University Hospital, Pauwelsstraβe 30, Aachen, Germany | [f] School of Cardiovascular Diseases (CARIM), University of Maastricht, Maastricht, Universiteitssingel 50, The Netherlands

Correspondence: [*] Corresponding author: Dr. Sarada D. Tetali, Associate Professor, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, Telangana, India. Tel.: +91 40 23134512; Fax: +91 40 23010120; E-mails: stetali@uohyd.ac.in; saradakanakagiri@gmail.com.

Abstract: Accumulated uremic toxins like indoxyl sulphate, hippuric acid and p-cresyl sulphates in renal failure patients stimulate proinflammatory effects, and consequently kidney and cardiovascular diseases. Low clearance rate of these uremic toxins from the blood of uremic patients by conventional techniques like hemodialysis is due to their strong covalent albumin binding (greater than 95%) and hydrophobic nature, which led to alternatives like usage of hydrophobic adsorber’s in removing these toxins from the plasma of kidney patients. Polymers like polyethylene, polyurethane, polymethylmethacrylate, cellophane and polytetrafluoroethylene were already in use as substitutes for metal devices as dialysis membranes. Among new synthetic polymers, one such ideal adsorber material are highly porous microparticles of poly(ether imide) (PEI) with diameters in the range from 50–180μm and a porosity around 88±2% prepared by a spraying and coagulation process. It is essential to make sure that these synthetic polymers should not evoke any inflammatory or apoptotic response during dialysis. Therefore in our study we evaluated in vitro effect of PEI microparticle extracts in human aortic endothelial cells (HEACs) concerning toxicity, inflammation and apoptosis. No cell toxicity was observed when HAECs were treated with PEI extracts and inflammatory/apoptotic markers were not upregulated in presence of PEI extracts. Our results ensure biocompatibility of PEI particles and further hemocompatibility of particles will be tested.

Keywords: Poly(ether imide) (PEI), biomaterial, inflammation, apoptosis, medical devices, Tumor necrosis factor-alpha(TNF-α)

DOI: 10.3233/CH-168046

Journal: Clinical Hemorheology and Microcirculation, vol. 64, no. 4, pp. 931-940, 2016