Affiliations: [a] Carlota Saldanha Lab, Instituto de Medicina Molecular, Universidade de Lisboa, Lisboa, Portugal
| [b] Instituto de Bioquímica Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal
| [c] Unidade de Cuidados Intensivos, Hospital Beatriz Ângelo, Loures, Portugal
Correspondence:
[*]
Corresponding author: Patrícia Napoleão, Carlota Saldanha Lab, Instituto de Medicina Molecular, Av. Professor Egas Moniz, 1649-028 Lisboa, Portugal. Tel.: +351 2199949; E-mail: pnapoleao@medicina.ulisboa.pt.
Abstract: AIM: Soluble CD40 ligand (sCD40L) has been considered as a marker of thrombosis and inflammation in several diseases, including sepsis. Recent studies challenge this view and point to a role of sCD40L in vascular and endothelial function. An indication of that association in sepsis has not been obtained so far. Therefore, herein we evaluated association between sCD40L and markers of hemorheology and inflammation on context of septic shock. METHODS: Time-changes of sCD40L levels over 72 hours of Intensive Care Unit (ICU) internment were assessed in 22 patients with septic shock and compared with 36 healthy volunteers. Association of sCD40L levels with erythrocyte deformability and aggregation (as markers of hemorheology), plasma concentrations of haemoglobin (Hb, as markers of endothelial function) and white blood cells (WBC) count (as marker of low-grade inflammation) were assessed in patients with septic shock. RESULTS: At ICU admission, sCD40L concentrations in patients with septic shock were lower (p = 0.024) than levels of healthy volunteers. However, sCD40L did not change over 72 hours of internment (F = 2.1, p = 0.137). Soluble CD40L levels in patients with septic shock at ICU admission correlate with concentrations of Hb (r = 0.61, p = 0.00) and WBC count (r = 0.63, p = 0.00), but not to erythrocyte deformability (r≥0.157, p≤0.235) and aggregation (r≥–0.109, p≤0.192). CONCLUSIONS: These results seem to highlight a possible association of sCD40L to endothelial function and inflammation in septic shock context.
