Article type: Research Article
Authors: Zheng, Qinni; | Long, Jinhua | Jia, Binbin | Xu, Xiaoli; | Zhang, Chunlin | Li, Long | Wen, Zongyao | Jin, Feng | Yao, Weijuan | Zeng, Zhu;
Affiliations: Department of Biotechnology, Guiyang Medical College, Guiyang, Guizhou Province, China | Department of Cell Biology, Guiyang Medical College, Guiyang, Guizhou Province, China | Department of Head and Neck, Affiliated Hospital and Cancer Hospital, Guiyang Medical College, Guiyang, Guizhou Province, China | Hemorheology Center, School of Basic Medical Sciences, Health Science Center of Peking University, Beijing, China | Department of Medical Physics, Chengde Medical College, Chengde, Hebei Province, China | Department of Nephrology, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou Province, China
Note: [] These authors contributed equally to this work.
Note: [] These authors contributed equally to this work.
Note: [] Corresponding author: Feng Jin, Department of Head and Neck, Affiliated Hospital and Cancer Hospital, Guiyang Medical College, Guizhou Province, China. Tel.: +86 851 6512802; Fax: +86 851 6520382; E-mail: Jinf8865@yahoo.com.cn
Note: [] Corresponding author: Weijuan Yao, Hemorheology Center, School of Basic Medical Sciences, Health Science Center of Peking University, Beijing, China. Tel.: +86 108 2802419; Fax: +86 108 2802419; E-mail: ywj01@hotmail.com
Note: [] Corresponding author: Zhu Zeng, Department of Biotechnology, Guiyang Medical College, Guiyang, Guizhou Province 550004, China. Tel.: +86 851 6909969; Fax: +86 851 6908828; E-mail: Zengzhu100@sina.com
Abstract: Dendritic cells (DCs) are potent and specialized antigen-presenting cells that play a crucial role in initiating and amplifying both the innate and adaptive immune responses. Tumor cells can escape from immune attack by secreting suppressive cytokines which solely or cooperatively impair the immune function and microrheological properties of DCs. However, the underlying mechanisms are not fully defined. Transforming growth factor-β1 (TGF-β1) has been identified as a major cytokine in the tumor microenvironment. To determine the effects of TGF-β1 on mature DCs (mDCs) from microrheological viewpoint, cells were treated with different concentrations of TGF-β1. The results showed that the impaired microrheological parameters, including osmotic fragility, electrophoretic mobility, deformability, membrane fluidity, F-actin organization and so on, as well as motilities of mDCs relied heavily on TGF-β1 concentration. Moreover, these changes were correlated with the expression levels of fascin1, cofilin1, phosphorylated cofilin1 and profilin, this could be one of the crucial aspects of immune escape mechanisms of tumors, hinting that the signal pathway of TGF-β1 should be blocked in appropriate way before performing DCs-based immunotherapy against cancer. It is clinically important to understand the biological behavior of DCs and immune escape mechanism of tumor as well as how to improve efficiency of the anti-tumor therapy based on DCs.
Keywords: Mature dendritic cells, transforming growth factor-$\beta_{1}$, microrheological characteristics, immune escape mechanism of tumor
DOI: 10.3233/CH-121653
Journal: Clinical Hemorheology and Microcirculation, vol. 56, no. 1, pp. 25-40, 2014
