Comparative erythrocyte deformability investigations by filtrometry, slit-flow and rotational ektacytometry in a long-term follow-up animal study on splenectomy and different spleen preserving operative techniques: Partial or subtotal spleen resection and spleen autotransplantation

Article type: Research Article

Authors: Miko, Iren^{a; 1} | Nemeth, Norbert^{a; 1; *} | Sogor, Viktoria^a | Kiss, Ferenc^a | Toth, Eniko^a | Peto, Katalin^a | Furka, Andrea^b | Vanyolos, Erzsebet^a | Toth, Laszlo^c | Varga, Jozsef^d | Szigeti, Krisztian^e | Benkő, Ilona^f | Olah, Anna V.^g | Furka, Istvan^a

Affiliations: [a] Department of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Debrecen, Hungary | [b] Department of Clinical Oncology, Division of Radiotherapy, Faculty of Medicine, University of Debrecen, Debrecen, Hungary | [c] Department of Pathology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary | [d] Department of Nuclear Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary | [e] Department of Biophysics and Radiation Biology, Faculty of Medicine, Semmelweis University, Budapest, Hungary | [f] Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Debrecen, Debrecen, Hungary | [g] Department of Laboratory Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary

Correspondence: [*] Corresponding author: Norbert Nemeth, MD, PhD, Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Nagyerdei krt. 98., Hungary. Tel./Fax: +36 52 416 915, E-mail: nemeth@med.unideb.hu.

Note: [1] These authors contributed equally this work.

Abstract: BACKGROUND: Partial or subtotal spleen resection or spleen autotransplantation can partly preserve/restore the splenic filtration function, as previous studies demonstrated. OBJECTIVE: For better evaluation and follow-up of the various spleen-preserving operative techniques’ effectiveness versus splenectomy, a composite methodological approach was applied in a canine experimental model. METHODS: Beagle dogs were subjected to control (n = 6), splenectomy (SE, n = 4), partial and subtotal spleen resection (n = 4/each) or spleen autotransplantation groups (AU, Furka’s spleen-chip method, n = 8). The follow-up period was 18 postoperative (p.o.) months. Erythrocyte deformability was determined in parallel by bulk filtrometry (Carat FT-1 filtrometer), slit-flow ektacytometry (RheoScan D-200) and rotational ektacytometry (LoRRca MaxSis Osmoscan). RESULTS: By filtrometry, relative cell transit time increased in the SE group (mostly in animal Nr. SE-3), showing the highest values on the 3rd, 9th and in 18th p.o. months. Elongation index values decreased in this group (both by slit-flow and rotational ektacytometers). In general, AU and two resection groups’ values were lower versus control and higher than in SE. CONCLUSIONS: Forasmuch in the circulation both elongation by shear stress and filtration occur, these various erythrocyte deformability testing methods together may describe better the alterations. Considering the possible complications related to functional asplenic-hyposplenic conditions, individual analysis of cases is highly important.

Keywords: Spleen filtration function, splenectomy, spleen autotransplantation, spleen partial or subtotal resection, red blood cell deformability, bulk filtrometry, slit flow and rotational ektacytometry

DOI: 10.3233/CH-160231

Journal: Clinical Hemorheology and Microcirculation, vol. 66, no. 1, pp. 83-96, 2017