Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Felthaus, Oliver* | Prantl, Lukas | Skaff-Schwarze, Mona | Klein, Silvan | Anker, Alexandra | Ranieri, Marco | Kuehlmann, Britta
Affiliations: Department of Plastic and Reconstructive Surgery, University Medical Center Regensburg, Regensburg, Germany
Correspondence: [*] Corresponding author: Oliver Felthaus, Department of Plastic and Reconstructive Surgery, University Medical Center Regensburg, Franz-Josef-Strauss-Allee 11, Bavaria, Regensburg 93053, Germany. Tel.: +49 941 944 4889; Fax: +49 941 944 6948; E-mail: oliver.felthaus@ukr.de
Abstract: BACKGROUND: Autologous fat grafts and adipose-derived stem cells (ASCs) can be used to treat soft tissue defects. However, the results are inconsistent and sometimes comprise tissue resorption and necrosis. This might be due to insufficient vascularization. Platelet-rich plasma (PRP) is a source of concentrated autologous platelets. The growth factors and cytokines released by platelets can facilitate angiogenesis. The simultaneous use of PRP might improve the regeneration potential of fat grafts. The optimal ratio has yet to be elucidated. A byproduct of PRP preparation is platelet-poor plasma (PPP). OBJECTIVE: In this study we investigated the influence of different concentrations of PRP on the vitality and differentiation of ASCs. METHODS: We processed whole blood with the Arthrex Angel centrifuge and isolated ASCs from the same donor. We tested the effects of different PRP and PPP concentrations on the vitality using resazurin assays and the differentiation of ASCs using oil-red staining. RESULTS: Both cell vitality and adipogenic differentiation increase to a concentration of 10% to 20% PRP. With a PRP concentration of 30% cell vitality and differentiation decrease. CONCLUSIONS: Both PRP and PPP can be used to expand ASCs without xenogeneic additives in cell culture. A PRP concentration above 20% has inhibitory effects.
Keywords: Adipose tissue derived stem cells, platelet rich plasma, tissue engineering
DOI: 10.3233/CH-160203
Journal: Clinical Hemorheology and Microcirculation, vol. 66, no. 1, pp. 47-55, 2017
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl