Article type: Research Article
Authors: Li, Yu-Juan | Han, Dong | Xu, Xiang-Shun | Liu, Yu-Ying | Sun, Kai | Fan, Jing-Yu | Qian, Rui-Qin | Han, Jing-Yan;
Affiliations: Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China | Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
Note: [] Corresponding author: Rui-Qin Qian, MD, Professor Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China. Tel.: +86 10 8280 2404; Fax: +86 10 8280 2996; E-mail: qianruiqin@bjmu.edu.cn
Note: [] Corresponding author: Jing-Yan Han, MD, PhD, Professor and Chairman, Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China. Tel.: +86 10 8280 2862; Fax: +86 10 8280 2996; E-mail: hanjingyan@bjmu.edu.cn
Abstract: This study intended to examine the effect of 3,4-dihydroxyphenyl lactic acid (DLA), a major ingredient of Salvia miltiorrhiza, on lipopolysaccahride (LPS) -induced mouse cerebral cortical microcirculatory disturbance. Velocity of red blood cells in, and albumin leakage from venules, and the numbers of leukocytes rolling on, and adherent to the venular wall were determined by an up-right microscope after LPS (5 mg/kg/h) infusion with or without administration of DLA (5 mg/kg/h). Expression of adhesion molecules CD11b/CD18 and L-selectin on neutrophils, plasma concentration of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were evaluated by flow cytometry. Concentration of TNF-α in supernatants of LPS-stimulated mononuclear cells was evaluated in vitro by flow cytometry as well. LPS exposure significantly increased the number of rolling and adherent leukocytes as well as albumin leakage, and decreased the velocity of red blood cells in venules. In addition, LPS stimulation apparently increased the expression of CD11b/CD18 on neutrophils, the concentration of plasma TNF-α, and the production of TNF-α from mononuclear cells. Treatment with DLA significantly ameliorated LPS-induced insults in mice, including cerebral microcirculatory disturbance, the expression of CD11b/CD18 on neutrophils, and the increased concentration of plasma TNF-α and the production of TNF-α from mononuclear cells.
Keywords: Brain, lipopolysacchride, TNF-α, vascular permeability, intravital microscopy
DOI: 10.3233/CH-2011-1435
Journal: Clinical Hemorheology and Microcirculation, vol. 50, no. 4, pp. 267-278, 2012
