CD14+ CD163+ IL-10+ monocytes/macrophages: Pro-angiogenic and non pro-inflammatory isolation, enrichment and long-term secretion profile
Issue title: Selected Papers from the 28th Congress on Clinical Hemorheology and Microcirculation of the German Society, Munich, Germany, 20–21 November 2009
Affiliations: Centre for Biomaterial Development, Institute for Polymer Research, GKSS Research Centre Geesthacht GmbH, Teltow, Germany | Berlin-Brandenburg Centre for Regenerative Therapies, Berlin, Germany
Note: [] Corresponding author: B. Hiebl, Centre for Biomaterial Development, Institute for Polymer Research, GKSS Research Centre Geesthacht GmbH, Kantstrasse 55, 14513 Teltow, Germany. Tel.: +49 3328 352 467; Fax: +49 3328 352 452; E-mail: bernhard.hiebl@gkss.de
Abstract: The establishment of a stable endothelial layer on a biomaterial suture is a well known strategy to achieve hemocompatibility. The endothelialisation is supported by factors as e.g. vascular endothelial growth factor (VEGF), which can be secreted by monocytes/macrophages (mo/mΦ) in an angiogenic milieu. In order to avoid detrimental inflammation triggered by these mo/mΦ, we established a protocol for the generation of alternatively activated macrophages and studied their response towards VEGF-A165. We could generate sufficient amounts of these CD14+ CD163+ IL-10+ mo/mΦ from buffy coats(8.6 ± 4.7 × 105 cells/mlbuffy coat). Furthermore, we achieved a VEGF-A165 secretion in the nanogram range. The VEGF-A165 secretion increased 2.1-fold within 14 days from 7.6 ± 2.2 to 16.1 ± 2.5ng/ml when the cells were grown with a VEGF-A165 supplemented (10ng/ml) cell culture medium. Within this time period the secretion levels of other pro-angiogenic growth factors (bFGF, PDGF-BB) and immunomodulatory cytokines (IL-10, IL-12, IL-1ra, TNFα, IFNγ) reached only the picogram range. These results suggest that angiogenically stimulated CD14+ CD163+ IL-10+ mo/mΦ might be useful as a cellular cytokine delivery system supporting endothelialisation of biomaterials without inducing pro-inflammatory effects.
