Affiliations: [a] Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China | [b] Department of Oncology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China
Correspondence:
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Corresponding author: Wen He, Department of Oncology, The Second Affiliated Hospital of Harbin Medical University, Xuefu Road No. 246, Nangang District, Harbin 150008, Heilongjiang, China. Tel.: +86 0451 86662961; E-mail:hewena_0426@163.com
Abstract: OBJECTIVE: To study the correlation between single nucleotide
polymorphism (SNP) of hsa-miR-124a and risk and prognosis of osteosarcoma (OS). METHODS: OS patients (n = 174) hospitalized at The Second
Affiliated Hospital of Harbin Medical University from January 2010 to March
2012 were selected as case group by inclusion and exclusion criteria, and
healthy people (n = 150) receiving physical examination at the same duration
were recruited as control group. Polymerase chain reaction-ligase detection
reaction (PCR-LDR) was performed for genotyping of hsa-miR-124a rs531564. RESULTS: There were significant differences in the frequency
distribution of genotypes and alleles of hsa-miR-124a rs531564 in the
case and control group (all P < 0.05); the individuals carrying with CG + GG
genotype showed significantly decreased risk for OS. The clinical pathological
characteristics were significantly different in the patients with CC
genotype and CG + GG genotype, including tumor size, tumor differentiation
grading, Enneking staging, operation manner, time of chemotherapy and
metastasis (all P < 0.05). The 5-year survival rate of the cases with CC
genotype was significantly lower than that of the ones with CG + GG genotype
(P < 0.05). CG + GG genotype, Enneking staging and operation manner were
independent risk factors for prognosis of OS (all P < 0.05). CONCLUSIONS: CG +$ GG genotype of hsa-miR-124a rs531564 had
decreased risk for OS and affected prognosis of OS.
