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Article type: Research Article
Authors: Radwan, Sara M.a | Hamdy, Nadia M.a | Hegab, Hany M.b | El-Mesallamy, Hala O.a; *
Affiliations: [a] Biochemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt | [b] Department of Internal Medicine-Clinical Hematology Unit, Faculty of Medicine, Ain Shams University, Cairo, Egypt
Correspondence: [*] Corresponding author: Hala O. El-Mesallamy, Biochemistry Department, Faculty of Pharmacy, Ain Shams University, Abassia 11566, Cairo, Egypt. E-mail:hala.elmesallamy@hotmail.com
Abstract: BACKGROUND: Beclin-1, an important autophagic gene, and hypoxia-inducible factor-1α (HIF-1α), the master regulator of the hypoxic response, are reported in several human cancers. However, their expressions in acute leukemia haven't yet been well investigated. OBJECTIVE: This study was designed to investigate the gene expression of beclin-1, microtubule-associated protein-1 light chain-3B (MAB1LC3B), the anti-apoptotic marker Bcl-2, and HIF-1α, as well as to evaluate the relationship between their expressions profile and prognosis in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) adult patients. METHODS: The study involved 30 AML patients, 25 ALL patients, and 20 controls. Gene expression was analyzed using quantitative reverse transcriptase polymerase chain reaction (QRT-PCR). RESULTS: In both AML and ALL groups, beclin-1 and MAB1LC3B expressions were significantly down-regulated (p < 0.001), while HIF-1α (p < 0.01) and Bcl-2 (p < 0.001) expressions were significantly up-regulated compared to the control group. HIF-1α fold expression was significantly negatively correlated with beclin-1 (p < 0.01). Moreover, decreased beclin-1 gene expression and increased HIF-1α gene expression were both associated with poor survival, supporting their pivotal role in the development and progression of acute leukemia. CONCLUSIONS: Both Beclin-1 and HIF-1α could be considered as important biomarkers determinants of pathogenesis and survival in acute leukemia.
Keywords: Acute leukemia, autophagy, Beclin-1, MAP1LC3B, HIF-1α
DOI: 10.3233/CBM-160603
Journal: Cancer Biomarkers, vol. 16, no. 4, pp. 619-626, 2016
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