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Article type: Research Article
Authors: Li, Zhen | Shu, Jinian* | Zhang, Peng | Sun, Wanqi | Yang, Bo | Zhang, Haixu
Affiliations: State Key Joint Laboratory of Environment Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China
Correspondence: [*] Corresponding author: Jinian Shu, State Key Joint Laboratory of Environment Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China. E-mail:jshu@rcees.ac.cn
Abstract: BACKGROUND: Identifying endogenous volatile organic compounds (VOCs) as markers for different cancers currently requires time-consuming procedures and specialized operators. OBJECTIVE: The objective of this study was to develop a rapid and simple method for measuring VOCs at trace levels. METHODS: A simple vacuum ultraviolet photoionization mass spectrometer (VUV-PIMS) was used to detect trace levels of dimethyl trisulfide (DMTS), dimethyl sulfide (DMS), and 2-butanone, which correspond to volatile biomarker candidates present in the exhaled breath of patients with breast, liver, and lung cancers, respectively. The practicality of measuring endogenous VOCs using VUV-PIMS was confirmed by detecting them in cultured cell lines. RESULTS: The abovementioned VOCs were detected with high sensitivity by VUV-PIMS. The limits of detection (LODs) for DMTS, DMS, and 2-butanone were 3.1, 3.9, and 23.2 pptv, respectively, under ambient conditions, which surpass the sensitivity of nearly all other MS-based techniques. Moreover, relatively high concentrations of 2-butanone and DMS were observed in VOCs emitted from the A549 lung cancer cell line and the HepG2 liver cancer cell line, respectively. CONCLUSIONS: Our results show that VUV-PIMS may serve as a reliable method for real-time measurement of endogenous volatile cancer biomarkers.
Keywords: VUV-PIMS, cancer, endogenous VOCs, cell measurement
DOI: 10.3233/CBM-160587
Journal: Cancer Biomarkers, vol. 16, no. 3, pp. 477-487, 2016
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