Affiliations: Department of Gastroenterology, Suzhou Municipal Hospital, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, Jiangsu, China
Correspondence:
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Corresponding author: Chunxiao Zhou, Department of Gastroenterology, Suzhou Municipal Hospital, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou 215002, Jiangsu, China. Tel.: +86 512 62362044; Fax: +86 512 62362512; E-mail:zhouchunxiao1126@163.com
Abstract: OBJECTIVE: The aim of the study was to evaluate whether the
insertion/deletion polymorphism (rs3783553) locating in the 3'
untranslated region (3'UTRs) of IL-1A was related to the risk of gastric cancer
(GC) in a Chinese population and explore the possible molecular mechanism. METHODS: Genomic DNA was extracted from peripheral venous blood of
519 GC patients and 536 healthy control individuals. The IL-1A rs3783553
polymorphism was genotyped by using a polymerase chain reaction assay. The
vectors containing the insertion or deletion allele were constructed, and
luciferase assay was used to detect the effect of the polymorphism on the
transcriptional activity of IL-1A. RESULTS: Strong evidence of association was observed between the
IL-1A rs3783553 polymorphism and susceptibility to GC in the study. In addition,
the `TTCA' insertion allele of rs3783553 disrupts the binding site for
miR-122 and miR-378, thereby increasing transcription of IL-1α in vitro. CONCLUSION: These findings suggest that functional polymorphism
rs3783553 in IL-1A could contribute to GC susceptibility, possibly or at least
partially through affecting the transcriptional activity of IL-1A.
Keywords: Insertion/deletion polymorphism, rs3783553, interleukin-1A, gastric cancer
