Subtitle:
Article type: Research Article
Authors: Khan, Mosin S.a | Pandith, Arshad A.b | Masoodi, Shariq R.c; d | Khan, Shoukat H.e | Rather, Tanveer A.e | Andrabi, Khursid I.f | Mudassar, Syeda; *
Affiliations: [a] Department of Clinical Biochemistry, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India | [b] Advanced Centre for Human Genetics, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India | [c] Department of Endocrinology, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India | [d] Department of Endocrinology, Diabetes and Nutrition, School of Medicine, University of Maryland, Baltimore, MD, USA | [e] Department of Nuclear Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India | [f] Department of Biotechnology, University of Kashmir, Srinagar
Correspondence:
[*]
Corresponding author: Syed Mudassar, Department of Clinical Biochemistry, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir 190011, India. Mobile: +91 9419016201; Tel.: +91 194 2400064, ext: 2206, 2381; E-mail: syed.mudassar@skims.ac.in
Abstract:
BACKGROUND: Among various polymorphic variants of TP53 gene, codon 72
polymorphism (Arg72Pro) has been found to be associated with cancer
susceptibility, but only few studies have investigated their effect on
thyroid cancer risk. OBJECTIVE: A case control study was conducted to elucidate the
possible role of this SNP as risk factor in thyroid cancer development and
to examine its correlation with various clinicopathological variables. METHODS: In this study, we tested the genotype distribution by
PCR-RFLP in 140 thyroid cancer patients and 200 cancer-free controls from
Kashmir Valley. RESULTS: Genotype frequencies of Arg/Arg (GG), Arg/Pro (GC), and
Pro/Pro (CC) genotypes among cases were 0.286, 0.343 and 0.371 while in
controls 0.45, 0.37 and 0.18 respectively. Proline allele frequency was
significantly higher than arginine frequency in patient group (OR = 2.06,
95% C.I = 1.5-2.8). Significant association was found between variant
genotype of codon 72 of TP53 gene and young age group, female gender, urban
dwellers, non-smokers and patients with elevated TSH levels (P < 0.05). CONCLUSION: It is evident from our study that Arg72Pro SNP of TP53 gene
is connected with higher susceptibility to thyroid cancer especially in
young age group, female gender, non-smokers and patients with elevated TSH
levels, hence, implicated in thyroid carcinogenesis.
Keywords: Papillary thyroid cancer, thyroid stimulating hormone, benign thyroid disease, differentiated thyroid cancer, polymerase chain reaction, restriction digestion
DOI: 10.3233/CBM-150485
Journal: Cancer Biomarkers, vol. 15, no. 4, pp. 459-465, 2015
