Affiliations: [a] The Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China | [b] Cancer Center, Traditional Chinese Medicine Integrated Hospital, Southern Medical University, Guangzhou, Guangdong, China | [c] Department of Medical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China
Correspondence:
[*]
Corresponding author: Haiyan Zheng, The Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510315, China. E-mail:ceozhy_1985@126.com
Abstract: Heparanase(HPSE), an endo-β -D-glucuronidase, is found overexpressed
in Ovarian cancer (OC). The purpose of our work was to investigate
primitively the possible role of HPSE in the development of OC. In this
study, RNA interference (RNAi) with a HPSE small hairpin RNAs(HPSE-shRNA)
and plasmid with HPSE were used to identify the effects of HPSE on the
regulation of malignant behaviors of OC. OV-90 and SKOV3 were selected as a
cell model in vitro and in vivo. The results showed that down-regulation of
HPSE can significantly inhibit the proliferative and invasive ability of
SKOV3 cells, and up-regulation of HPSE in OV-90 cells showed the opposite
effects. Compared with the parental OC cells, HPSE silencing cells exhibited
attenuated capacities in developing tumor in nude mice, while the growth
tumors xenografts derived from these cells were dramatically regressed. In
conclusion, our results suggest that HPSE contributes to the proliferation
and metastasis of OC and HPSE might be a potent molecular target for OC
treatment.
