Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Vingiani, Andreaa; b; 1 | Lorenzini, Danielea; 1 | Conca, Elenaa; 1 | Volpi, Chiara Costanzaa | Trupia, Desirè Violaa | Gloghini, Annunziataa | Perrone, Federicaa | Tamborini, Elenaa | Dagrada, Gian Paoloa | Agnelli, Lucaa | Capone, Iolandaa | Busico, Adelea; 2 | Pruneri, Giancarloa; b; 2; *
Affiliations: [a] Department of Diagnostic Innovation, Foundation IRCCS National Cancer Institute, Milan, Italy | [b] Department of Oncology and Hematoncology, University of Milan, Milan, Italy
Correspondence: [*] Corresponding author: Giancarlo Pruneri, Department of Diagnostic Innovation, Foundation IRCCS National Cancer Institute, 20133, Milan, Italy. Tel.: +39 223902260; E-mail: giancarlo.pruneri@istitutotumori.mi.it.
Note: [1] These authors contributed equally to this work.
Note: [2] Contributed equally to this work.
Abstract: BACKGROUND: Pan-TRK inhibitors Entrectinib and Larotrectinib have been recently approved as tumor-agnostic therapies in NTRK1-2-3 rearranged patients and there is therefore an urgent need to identify reliable and accessible biomarkers for capturing NTRK fusions in the real-world practice. OBJECTIVE: We aim to assess the analytical validity of the recently released pan-TRK assay (Ventana), running a head-to-head comparison between immunohistochemistry and Archer FusionPlex Lung Panel (ArcherDX) that is designed to detect key fusions in 13 genes, also including NTRK1-3. METHODS: Pan-TRK IHC and NGS analysis were conducted on a retrospective/prospective cohort of 124 cancer patients (carcinomas, 93 cases; soft tissue sarcomas, 19; primary central nervous system tumours, 10; and neuroblastomas, 2). FISH data were available in most of the IHC/NGS discordant cases. RESULTS: A comparison between IHC and NGS results was carried out in 117 cases: among 30 pan-TRK positive cases, NTRK rearrangement by NGS was found in 11 (37%), while one of the 87 (1.1%) pan-TRK negative cases (a case of NSCLC) showed a TPM3-NRTK1 rearrangement by NGS. Accordingly, sensitivity and specificity of IHC in predicting NTRK status were 91.7% and 81.9%, respectively, while negative (NPV) and positive predictive value (PPV) were 98.8% and 36.7%, respectively. CONCLUSIONS: These data lead to suggest that IHC with VENTANA pan-TRK antibody can be a reliable screening tool for the identification of patients potentially bearing NTRK rearranged tumours.
Keywords: NTRK, next-generation sequencing, immunohistochemistry, Fluorescent in Situ Ibridization, Biomarker
DOI: 10.3233/CBM-220357
Journal: Cancer Biomarkers, vol. 38, no. 3, pp. 301-309, 2023
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl