Affiliations: [a] Department of Science Education, Korea National University of Education, Cheongju-si, Chungbuk, Republic of Korea | [b] Department of Biology Education, Korea National University of Education, Cheongju-si, Chungbuk, Republic of Korea
Correspondence:
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Corresponding author: Wanyeon Kim, Department of Biology Education, Korea National University of Education, 250 Taeseongtabyeon-ro, Gangnae-myeon, Heungdeok-gu, Cheongju-si, Chungbuk 28173, Republic of Korea. Tel.: +82 43 230 3750; E-mail: wykim82@knue.ac.kr.
Note: [1] The abstract of this article was published in the FASEB Journal under same title: Chae Y., Seo D., Kim D. and Kim W. Gene Expression Profiling after LINC00472 Overexpression in an NSCLC Cell Line. The FASEB Journal. 2021;35(S1). https://doi.org/10.1096/ fasebj.2021.35.S1.04918.
Note: [2] Authors contributed equally.
Abstract: Lung cancer accounts for a large proportion of cancer-related deaths worldwide. Personalized therapeutic medicine based on the genetic characteristics of non-small cell lung cancer (NSCLC) is a promising field, and discovering clinically applicable biomarkers of NSCLC is required. LINC00472 is a long non-coding RNA and has been recently suggested to be a biomarker of NSCLC, but little is known of its mechanism in NSCLC. Thus, the current study was performed to document changes in gene expression after LINC00472 overexpression in NSCLC cells. As a result of cell viability and migration assay, LINC00472 downregulated cell survival, proliferation, and motility. Transcriptome sequencing analysis showed 3,782 genes expression were changed in LINC00472 overexpressing cells. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed most genes were associated with intracellular metabolism. The PPP1R12B, RGS5, RBM5, RBL2, LDLR and PTPRM genes were upregulated by LINC00472 overexpression and these genes functioned as tumor suppressors in several cancers. In contrast, SPSB1, PCNA, CD24, CDK5, CDC25A, and EIF4EBP1 were downregulated by LINC00472, and they functioned as oncogenes in various cancers. Consequently, the function of LINC00472 in tumorigenesis might be related to changes in the expressions of other oncogenes and tumor suppressors.
Keywords: Long non-coding RNA LINC00472, transcriptome analysis, lung tumorigenesis
