Affiliations: Department of Geriatrics, Ruijin Hospital, Shanghai Jiaotong University, Shanghai, China
Correspondence:
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Corresponding authors: Zhi-Hong Xu, Department of Geriatrics, Ruijin Hospital, Shanghai Jiaotong University, 197 Ruijin Second Road, Huangpu District, 200025, Shanghai, China. Tel.: +86 13916707605; Fax: +86 21 54198967; E-mail: zhihxu@163.com. Jia-An Hu, Department of Geriatrics, Ruijin Hospital, Shanghai Jiaotong University, 197 Ruijin Second Road, Huangpu District, 200025, Shanghai, China. Tel.: +86 13601737014; Fax: +86 21 54198967; E-mail: hja10310@rjh.com.cn.
Note: [1] These authors contributed equally to this work as the co-first authors.
Abstract: Circular RNA (circRNA) has been shown to participate in various tumors, including lung cancer. In the present study, we explored the expression and functional relevance of hsa_circ_0003288 in human non-small cell lung cancer (NSCLC). We verified that hsa_circ_0003288 expression was upregulated in lung cancer tissues and cell lines. Overexpression of hsa_circ_0003288 dramatically promoted lung cancer cell proliferation, colony formation, inhibited apoptosis, and increased cell migration and invasion in vitro. Xenograft experiments showed that hsa_circ_0003288 overexpression accelerated tumor growth in vivo. Mechanistically, hsa_circ_0003288 negatively regulated miR-145 to exert the oncogenic role in lung cancer. Overexpression of miR-145 decreased cell proliferation, induced apoptosis, and suppressed migration and invasion in lung cancer. Additionally, miR-145 co-transfection abolished the oncogenic role of hsa_circ_0003288. Collectively, these findings identified a novel regulatory role of hsa_circ_0003288/miR-145 axis in the progression of NSCLC.
