Affiliations: Department of Breast Medical, Linyi Cancer Hospital, Linyi, Shandong 276002, China
Correspondence:
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Corresponding author: Xinglong Cai, Department of Breast Medical, Linyi Cancer Hospital, No. 8 Lingyuan Qian Street, Lanshan District, Linyi, %****␣cbm-27-cbm190530_temp.tex␣Line␣25␣**** Shandong 276002, China. Tel.: +86 539 812 1800; E-mail: iktk1fc9058774@163.com.
Abstract: BACKGROUND: Breast cancer has become a major threat to women’s physical and mental health. Many long noncoding RNAs (lncRNAs) have been reported to exert effect in the progression of breast cancer. However, the regulatory mechanism of HAND2-AS1 remains unclear. Therefore, this study aimed to elucidate the role of HAND2-AS1 in breast cancer. METHODS: The expression of HAND2-AS1, miR-1275 and SOX7 was assessed using RT-qPCR and Western blot analysis. CCK-8, Transwell and luciferase reporter assays were used to investigate their regulatory mechanisms. RESULTS: Downregulation of HAND2-AS1 was detected in breast cancer and was associated with adverse clinical features and prognosis. Furthermore, overexpression of HAND2-AS1 restrained cell viability, migration and invasion in breast cancer. In addition, reciprocal suppression between HAND2-AS1 and miR-1275 was identified in breast cancer cells. Further, SOX7, a target of miR-1275, strengthened the effect of HAND2-AS1 in breast cancer. CONCLUSION: LncRNA HAND2-AS1 sponging miR-1275 restrains proliferation and metastasis of breast cancer cells by regulating SOX7 expression.
Keywords: HAND2-AS1, breast cancer, miR-1275, SOX7
