Affiliations: [a] Department of Colorectal Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China | [b] Department of Clinical Laboratory, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China | [c] Department of Pathology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China | [d] Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China
Correspondence:
[*]
Corresponding authors: Yun Qian, Department of Clinical Laboratory, The Second Affiliated Hospital, Zhejiang University School of Medicine 88 Jiefang Road, Hangzhou, Zhejiang 310009, China. Tel.: +86 571 87783948; E-mail: qianyun1985@zju.edu.cn; Xiangming Xu, Department of Colorectal Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine 79 Qingchun Road, Hangzhou, Zhejiang 310003, China. Tel.: +86 571 87236582; E-mail: xuxiangming@zju.edu.cn.
Note: [1] Xu and Xiao contributed equally to this article.
Abstract: BACKGROUND:There were no specific indicators for the early detection of pancreatic cancer. OBJECTIVE:To analyze the diagnostic and prognostic value of CA19-9 plus B7-H4 detection in preoperative serum or surgical tissues of patients with pancreatic cancer. METHODS: One hundred and eighty-eight patients with pancreatic cancer and 25 controls were recruited. Their preoperative serum CA19-9 level was detected chemiluminescently, and B7-H4 expression in pancreatic cancer tissues was assessed immunohistochemically. The diagnostic and prognostic utility of detecting CA19-9, B7-H4 and their combination was evaluated. RESULTS:CA19-9 and B7-H4 levels were significantly upregulated in patients with pancreatic cancer compared with those in controls. The diagnostic value of combined CA19-9 plus B7-H4 detection was markedly better than that achieved from their separate detection analyzed with receiver operating characteristic curve. Sensitivity and specificity of the combined detection in the pancreatic cancer group was significantly increased compared with single detection. B7-H4 detection showed better prognostic value than detection of CA19-9. However, CA19-9 had high sensitivity and low specificity, while B7-H4 showed the opposite. The sensitivity of the combined prognosis was not significantly different to B7-H4 alone. CONCLUSION:The combined detection of CA19-9 and B7-H4 could become a new method for the clinical diagnosis and prognosis of pancreatic cancer.
