Potential diagnostic and prognostic value of plasma long noncoding RNA LINC00086 and miR-214 expression in gastric cancer

Article type: Research Article

Authors: Ji, Bing^{a; 1} | Huang, Youmin^{b; 1} | Gu, Ting^c | Zhang, Li’e^d | Li, Guohong^{e; *} | Zhang, Changgeng^{f; *}

Affiliations: [a] Zaozhuang Hospital of Zaozhuang Mining Group, Zaozhuang, Shandong, China | [b] Blood Transfusion Department, Tengzhou Central People’s Hospital, Tengzhou, Shandong, China | [c] First Affiliated Hospital of Xinjiang Medical University, Urumchi, Xinjiang, China | [d] Ningjin People’s Hospital, Dezhou, Shandong, China | [e] Laboratory Medicine, Dezhou Municipal Hospital, Dezhou, Shandong, China | [f] People’s Hospital of Hengshui City, Hengshui, Hebei, China

Correspondence: [*] Corresponding authors: Guohong Li, Dezhou Municipal Hospital, No. 18 Sanba Westroad Road, Dezhou 253012, Shandong, China. Tel.: +86 13969243766; E-mail: 13717687520@163.com; Changgeng Zhang, People’s Hospital of Hengshui City, Hengshui, Hebei, China. Tel.: +86 18003188030; E-mail: 13831839080@163.com.

Note: [1] Equal contributors.

Abstract: BACKGROUND:Plasma carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9), and cancer antigen 72-4 (CA72-4) are common markers which are useful in the diagnosis and prognosis of GC. However, their sensitivity and specificity in GC remain unsatisfactory. Identification of cancer diagnosed-biomarkers would be of great value. OBJECTIVE: Evaluate the diagnostic and prognostic value of LINC00086 and miR-214 in GC. METHODS:In this study, we determined the expression of LINC00086 and miR-214 in GC by qRT-PCR. Additionally, we investigated the relationship between various clinicopathological features of GC patients and LINC00086 or miR-214 expression, and evaluated the diagnostic and prognostic value of LINC00086 and miR-214 in GC. RESULTS: In this study, we found that plasma LINC00086 expression was significantly lower, whereas plasma miR-214 expression was significantly higher in GC patients than in normal individuals. LINC00086 and miR-214 exhibited high sensitivity and specificity in diagnosing GC. Additionally, GC patients with low LINC00086 or high miR-214 expression were likely to have larger tumors, lymphatic metastasis, larger TNM stage, and higher CEA and CA19-9 levels. Moreover, GC patients with low LINC00086 or high miR-214 expression showed lower survival rates. Lymphatic metastasis, LINC00086, and miR-214 are independent factors affecting patient diagnosis. CONCLUSIONS: LINC00086 and miR-214 are potentially diagnostic and prognostic markers for GC.

Keywords: Gastric cancer, LINC00086, miR-214, diagnosis, prognosis

DOI: 10.3233/CBM-181486

Journal: Cancer Biomarkers, vol. 24, no. 2, pp. 249-255, 2019