Affiliations: [a] Cancer Gene Therapy Research Center (CGRC), Zanjan University of Medical Sciences, Zanjan, Iran | [b] Student Research Committee, Zanjan University of Medical Sciences, Zanjan, Iran | [c] Department of Medical Genetics and Molecular Medicine, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran | [d] Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Correspondence:
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Correspondence: Mitra Khalili, Department of Medical Genetics and Molecular Medicine, School of Medicine, Mahdavi Blvd, Shahrak-e Karmandan, Zanjan, 45139-56111, Iran. Tel.: +98 243344337; Fax: +98 2433449553; E-mail: khalili.mitra@gmail.com,m.khalili@zums.ac.ir.
Abstract: BACKGROUND: Gastric cancer (GC) is the third leading cause of cancer-related death in the world. Dysfunction of long noncoding RNAs (lncRNAs) in cancers, especially those with role in pluripotency, are approved by increasing evidence. OBJECTIVE:SOX2 overlapping transcript (SOX2OT) lncRNA, is aberrantly expressed in different cancers; however its role in gastric cancer is still controversial. MATERIALS AND METHODS: In this study, the expression of SOX2OT was evaluated in 33 matched pair tumor and non-tumor gastric samples and AGS and MKN45 gastric and NTERA2 embryonic carcinoma cell lines by real time PCR. RESULTS: Our finding revealed a significant decrease in the expression of SOX2OT in gastric tumor samples compared to their matched non-tumor samples (P= 0.05) and also a lower expression in high grade compared to low grade of gastric malignancy. As we expected SOX2OT expression showed higher expression in NT2 compared to AGS and MKN45 cell lines. CONCLUSION: Simultaneous expression of SOX2 and SOX2OT was reported in some cancers. Regarding to the decreased expression of SOX2OT in the present study in concurrent with downregulation of SOX2 in our previous study, it seems that SOX2OT plays a tumor suppressor role in GC and may be useful biomarker for diagnosis of GC.
Keywords: Long Non Coding RNA, SOX2OT, Gastric Cancer, SOX2
