Affiliations: [a] Department of Oncology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China | [b] Department of Pathology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China | [c] Department of Radiology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China
Correspondence:
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Corresponding authors: Yan Zhou, Department of Radiology, the Affiliated Yixing Hospital of Jiangsu University, 75 Tongzhenguan Road, Wuxi, Jiangsu 214200, China. Tel.: +86 0510 87330802; Fax: +86 0510 87330802; E-mail: dryzhou@163.com;
Zhihong Cao, Department of Radiology, the Affiliated Yixing Hospital of Jiangsu University, 75 Tongzhenguan Road, Wuxi 214200, China. E-mail: luoyifeng1207@163.com
Abstract: BACKGROUND: Cullin1 and MMP-2 have been identified as important
markers in various cancers, but their roles in colorectal cancer (CRC) have
remained to be discovered. The aim of this study was to investigate the
expression pattern and significance of Cullin1 and MMP-2 in CRC.
METHODS: A total of 470 CRC patients were enrolled. Archival
paraffin-embedded CRC tissue samples were used to generate tissue microarray
blocks, which were immunohistochemically stained for Cullin1 and MMP-2.
Prognostic and predictive role of Cullin1 and MMP-2 expression was evaluated
by univariate and multivariate analysis, respectively. Values of p < 0.05
were considered statistically significant.
RESULTS: Cullin1 and MMP-2 protein levels were significantly
upregulated in CRC tissues compared with adjacent noncancerous tissues. High
tumoral Cullin1 or MMP-2 expression significantly correlated with shorter
overall survival (OS), as well as with clinicopathologic characteristics in
patients. Multivariate regression analysis showed that high Cullin1 and
MMP-2 expressions, separately and together, were independent negative
markers of OS.
CONCLUSION: Cullin1 and MMP-2 expressions could be novel diagnostic
and prognostic markers for CRC patients.
