Affiliations: [a] Department of Clinical Chemistry, The Second Hospital of Shandong University, Jinan, Shandong, China | [b] Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China | [c] Department of Orthopedics, The People's Hospital of Penglai City, Penglai, Shandong, China | [d] Department of Orthopedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China
Correspondence:
[*]
Corresponding author: Yong-Kui Zhang, Department of Orthopedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, 16,369 Jingshi Road, Jinan 250014, Shandong, China. E-mail:xiaoniu0928@163.com
Note: [1] Contributed equally to the work.
Abstract: BACKGROUND: As one of the endoplasmic reticulum proteins, calreticulin (CRT) plays a significant role in the body, and it has been used by many
researchers as a target for anti-tumor therapy. OBJECTIVE: The main purpose of the present study was to study expression of CRT of human osteosarcoma, and analyze the distinctions between normal and
tumor tissues, pre- and post-chemotherapy patients, and metastatic and
non-metastatic tumors in respect to this expression. METHODS: Immunofluorescent staining was used in order to investigate
expression of CRT in diverse tissues. The whole RNA and proteins were
extracted from the crushed tissues and used in the reverse transcription
polymerase chain reaction (RT-PCR) and western blotting analysis. RESULTS: The present study detected expression of CRT in patients with
osteosarcoma and revealed a higher expression level in normal tissues
surrounding tumors compared with tumor tissues, in the non-metastasis group
compared with the metastasis group, and in the chemotherapy group compared
with the non-chemotherapy group. CONCLUSIONS: These results could indicate a brand-new biological marker
which may be applied to estimate the features and prognosis of osteosarcoma.
Keywords: Osteosarcoma, Calreticulin, immunofluorescent staining, reverse transcription polymerase chain reaction, western blot analysis
