Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Huang, Jiea; 1 | Gao, Chunf; 1 | Ding, Xilaic; 1 | Qu, Shoufanga; e; 1 | Liu, Lichengd | Wu, Weilib | Zhang, Lininge | Zhao, Jinyinb; * | Gao, Shangxiana; *
Affiliations: [a] Department of In Vitro Diagnostic Reagents and Medium, National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China | [b] Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China | [c] Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China | [d] Beijing BGI-GBI Biotech Co., Ltd, Beijing Airport Industrial Zone B-8, Beijing, China | [e] Department of Immunology, Shandong University School of Medicine, Jinan, Shandong, China | [f] Key Laboratory of Clinic Immunology of Jiangsu Province, Department of Laboratory Medicine, First Hospital Affiliated to Soochow University, Suzhou, Jiangsu, China
Correspondence: [*] Corresponding authors: Shangxian Gao, Department of In Vitro Diagnostic Reagents and Medium, National Institute for the Control of Pharmaceutical and Biological Products, No.2, Tiantan Xili, Dongcheng District, Beijing, China. Tel.: +86 10 67095647; E-mail: gaoshangxian@yahoo.cn; Jinyin Zhao, Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, No.7 Beitucheng West Road, Chaoyang District, Beijing 100029, China. Tel.: +86 10 82275435.
Note: [1] These authors contributed equally to this work.
Abstract: Aim:For full-scale analysis of Human Papillomavirus (HPV) status in humans, two minor groove binder (MGB)-based one-step multiplex real-time PCR systems were developed: one to screen 16 high-risk HPV (HR-HPV) types, and one to screen a broader spectrum of HPV types (common HPV or C-HPV). Methods:Sensitivity and specificity were evaluated using diluted reference plasmids and 20 control human DNA samples. For clinical evaluation, 510 cervical scrape samples were evaluated. Results:The sensitivity assays revealed that the C-HPV detection system could detect 10 ∼ 100 plasmid copies/reaction, while the HR-HPV detection system detected 10 ∼ 500 copies. The specificity test revealed that the systems did not yield positive signals from the 20 human genomic DNA samples. Performance was tested on 510 usable clinical samples. The HR-HPV results were compared to those from the Hybrid Capture 2 (HC2) test, which assesses 13 HR-HPV types; the concordance level between the two methods was 90.8% with a kappa value of 0.813. Conclusions:These results showed that our novel MGB-based one-step multiplex real-time PCR method may be used for the diagnosis and mass screening of HPV in clinical and large-scale epidemiological studies.
Keywords: HPV, MGB, multiplex detection
DOI: 10.3233/CBM-130298
Journal: Cancer Biomarkers, vol. 12, no. 3, pp. 107-113, 2013
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl