Affiliations: [a] Department of Cytopathology and Gynecologic Pathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India | [b] Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India | [c] Department of General Surgery, Postgraduate Institute of Medical Education and Research, Chandigarh, India
Correspondence:
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Corresponding author: Gupta Nalini, Department of Cytopathology and Gynecologic Pathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India. Tel.: +91 172 2755114; Fax: + 91 172 2744401; E-mail: nalini203@gmail.com
Abstract:
BACKGROUND: Breast tuberculosis (TB) is an uncommon condition even in endemic countries.
Breast abscess is usually seen in young females. PCR helps in such
challenging cases to clinch the correct diagnosis. OBJECTIVE: The present study was performed to compare FNAC and TB PCR in inflammatory
lesions of breast. METHODS: FNAC cases reported as breast abscess, necrosis, non-specific and
granulomatous inflammation on cytology was taken. The material for PCR was
obtained from archived MGG smears and PCR for Mycobacterium tuberculosis for insertion sequence IS6110 was performed. RESULTS: A total of 54 cases were studied. The age ranged from 19-55 years. On FNAC,
acute suppurative inflammation without granulomas and negative AFB staining
was noted in 18 cases; granulomatous inflammation/mastitis in 26 cases and
granulomatous inflammation with necrosis with AFB positivity confirming
tuberculosis in 10 cases. PCR for Mycobacterium tuberculosis for insertion sequence IS6110 was
performed. None of the 18 cases of acute suppurative inflammation had
positivity for PCR. 13/26 [53.3%] cases of granulomatous inflammation/
mastitis with AFB negativity were positive for PCR. 10/10 cases of
granulomatous inflammation with necrosis with AFB positivity showed PCR
positivity. Therefore, proven cases of TB after FNAC were only
10/54 [17.24%] and the sensitivity increased to 23/54 [42.6%] after
performing PCR on FNA aspirate. CONCLUSION: PCR helped in identifying 13/26 [50%] cases reported as granulomatous
inflammation on cytology as tuberculosis. FNAC acted as an efficient
modality for collection of material for PCR. This study shows how PCR aids
in diagnosis of breast TB.
