Affiliations: Department of Medicine, University of Pécs, Pécs, Hungary | Department of Physiology and Biophysics, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA | Department of Pediatrics, Children's Hospital Los Angeles, Los Angeles, CA, USA | Department of Physics and Astronomy, University of Southern California, Los Angeles, CA, USA
Note: [] Address for correspondence: Prof. Jack Feinberg, Department of Physics and Astronomy, SSC 327, University of Southern California, Los Angeles, CA 90089-0484, USA. Tel.: +1 213 740 1134; Fax: +1 213 740 7886; E-mail: feinberg@usc.edu
Abstract: Ektacytometry measures the shape of red blood cells under shear stress by analyzing the diffraction pattern of laser light passing through a thin layer of suspended cells. Here we model the diffraction pattern using a combination of Bessel and anomalous scattering functions, and employ a global pattern-fitting technique over nine different shear stresses to determine the separate mechanical properties of normal and non-deformable cells. This technique is capable of yielding the correct elongation index of the normal cells over a range of shear stresses even when they are mixed with as much as 50% non-deformable cells. Additionally, the relative concentrations of normal and non-deformable cells can be determined.
Keywords: Cell analysis, medical and biological imaging, light scattering
DOI: 10.3233/BIR-2012-0616
Journal: Biorheology, vol. 49, no. 5-6, pp. 317-328, 2012
