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Article type: Research Article
Authors: Matsui, Tsubasa S.; | Deguchi, Shinji | Sakamoto, Naoya | Ohashi, Toshiro; | Sato, Masaaki;
Affiliations: Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University, Sendai, Japan | Department of Biomedical Engineering, Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan | Present address: Division of Human Mechanical Systems and Design, Graduate School of Engineering, Hokkaido University, Sapporo, Japan
Note: [] Address for correspondence: Tsubasa S. Matsui, Tohoku University, 6-6-11-1306-2 Aramaki-Aoba, Sendai 980-8579, Japan. Tel./Fax: +81 22 795 3936; E-mail: tsubasa@bml.mech.tohoku.ac.jp.
Abstract: Conventional atomic force microscopy is one of the major techniques to evaluate mechanical properties of cells and subcellular components. The use of a cantilever probe for sample manipulation within the vertical plane often makes absolute positioning of the probe, subject to thermal drift, difficult. In addition, the vertical test is unable to observe changes in the sample structure responsible for mechanical behavior detected by the probe. In the present study, an alternative mechanical tester was developed that incorporated a pair of micro-needles to manipulate a sample in a project plane, allowing acquisition of the accurate probe position and entire sample image. Using a vision-based feedback control, a micro-needle driven by a piezo actuator is moved to give user-defined displacements or forces to sample. To show its usefulness and versatility, three types of viscoelastic measurements on actin stress fibers isolated from smooth muscle cells were demonstrated: strain rate-controlled tensile tests, relaxation tests and creep tests. Fluorescence imaging of the stress fibers using Qdots over the course of the measurements, obtained through multiple image detectors, was also carried out. The technique described here is useful for examining the quantitative relationship between mechanical behavior and related structural changes of biomaterials.
Keywords: Tensile tester, visual feedback, fluorescence microscopy, cytoskeleton, stress fiber, viscoelastic properties
DOI: 10.3233/BIR-2009-0551
Journal: Biorheology, vol. 46, no. 5, pp. 401-415, 2009
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