Affiliations: Department of Mechanical Engineering and Mechanics, Drexel University, 3141 Chestnut St., STE 2‐115, Philadelphia, PA 19104, USA
Note: [] Address for correspondence: David Wootton, Dept. of Mechanical Engineering and Mechanics, Drexel University, 3141 Chestnut St, STE 2‐115, Philadelphia, PA 19104, USA. Fax: 215 895 1478; E‐mail: dwootton@coe.drexel.edu.
Abstract: Platelet margination (enhanced platelet concentration in the near wall region of a blood vessel) has been well documented in small vessels. In artery‐sized vessels margination has only been demonstrated in one study, using ghost cell suspensions and under relatively non‐physiologic conditions of steady flow and 50 cm development length. Local sampling experiments were performed to confirm platelet margination in artery‐sized stainless steel tubes, for a typical anatomical length and under pulsatile flow, using fresh EDTA‐anticoagulated porcine whole blood (N=21). Experiments were designed using three‐dimensional Computational Fluid Dynamics (CFD) to model the sample region with greater fidelity. Steady flow experiments in 50 cm long tubes verify published laser Doppler measurements of platelet margination in 3 mm ID tubes at normal arterial shear rate (500 s−1). Margination persists under pulsatile flow conditions (63.8 pulses/min), but in steady flow at length of 10 cm, margination is reduced. Platelet margination ratio (the ratio of the platelet concentration near the wall to bulk average platelet count) ranges from 1.21 to 2.48. No significant effects of calculated sampling thickness (20 μm and 50 μm) or pulsatility were detected. Hematocrit margination ratio is 0.68 to 0.90. Two model platelet concentration profiles are fit to the experimental results.
Keywords: Margination, platelet concentration, local sampling, porcine model, thrombosis
Journal: Biorheology, vol. 41, no. 2, pp. 113-125, 2004
