Visible spectroscopic technique for flowing erythrocytes in capillary

Issue title: Proceedings of the Seventh International Congress of Biorheology. Part I. Palais des Congrès, Nancy, France, 18–23 June 1989. Dedicated to Richard Skalak

Guest editors: Alfred L. Copley and Jean-François Stoltz

Article type: Research Article

Authors: Shiga, Takeshi^b | Tateishi, Norihiko^a | Maeda, Nobuji^a

Affiliations: [a] Department of Physiology, School of Medicine, Ehime University, Ehime 791-02, Japan | [b] Department of Physiology, School of Medicine, Osaka University, Osaka 530, Japan

Note: [] Accepted by: Guest Editor T. Koyama

Abstract: An optical spectroscopic system for determining the rate of oxygen release from flowing erythrocytes in microvessel is developed. The apparatus consists of following units attached to an inverted microscope. 1)A scanning spectrophotometer, equipped with a grating and a photon counter, was connected to an eyepiece of the microscope through a narrow light-guide, as to obtain the absorption spectrum (wave length range: 450 – 650 nm) of a focused spot ( ϕ = 7 μm).2)The velocity of erythrocyte flow was measured by dual-spots cross-correlation method, using two photomultipliers (connected to AID converter and microcomputer) with two light-guides inserted into another eyepiece.3)The diameter of vessel was estimated from digitized video-images, using a color image-processor. The ability of the apparatus was tested with (a) hemoglobin solution, (b) flowing erythrocyte suspension and (c) capillaries of rat mesentery. The rate of oxygen release through the vessel wall was calculated.

Keywords: Absorption spectrum, Capillary, Erythrocytes, Hemoglobin, Oxygen release

DOI: 10.3233/BIR-1990-273-415

Journal: Biorheology, vol. 27, no. 3-4, pp. 389-397, 1990