A freeze-capture method for tile study of platelet-sized particle distributions

Article type: Research Article

Authors: Bilsker, David L. | Waters, Christopher M. | Kippenhan, J. Shane | Eckstein, Eugene C.

Affiliations: Department of Biomedical Engineering, University of Miami, Coral Gables, FL 33124, U.S.A.

Note: [] Accepted by: Editor H.L. Goldsmith

Abstract: A rapid freezing method was developed to study the distributions of fluorescent platelet-sized particles in flows of blood suspensions through thin-walled capillary tubes. Segments of frozen tubes were mounted in a refrigerated microtome on the stage of an epifluorescence microscope. Sections of tube were cut away, images of newly exposed cross-sections were recorded on video tape, and distances of the particles from the wall were measured from recorded images. The distance data were used to construct histograms that were proportional to the local concentration. Results indicated that this method is suitable for the study of the distribution of platelet-sized particles over a wide range of hematocrit, that the basic profile is reproducible to within 15%, and that the non-uniform profile is not a result of events at the tube entrance.

Keywords: Erythrocytes, Platelets, Rheology, Biological Transport

DOI: 10.3233/BIR-1989-26606

Journal: Biorheology, vol. 26, no. 6, pp. 1031-1040, 1989