Experimental study on filtrability of polymorphonuclear leukocyte suspensions

Article type: Research Article

Authors: Yamaguchi, Saburo | Niimi, Hideyuki

Affiliations: National Cardiovascular Center Research Institute, Suita, Osaka 565, Japan

Note: [] Accepted by: Editor E. Fukada

Abstract: Filtrability of a suspension of polymorphonuclear leukocytes (PMNs) was examined in a Nuclepore membrane filtration system utilizing a gradually reduced pressure difference with or without an additional negative pressure. The filtration process was continuously recorded using a TV-video system for data analysis. The PMN content in the filtrate was directly measured. The pressure-flow relation was analyzed in terms of the relative resistance of the PMN suspension to that of the suspending medium. The relative resistance of the PMN suspension increased with an increase in the filtered volume until it approached infinity at the level of low pressure difference (2.8 - 0 cmH2O). The remarkable increase in flow resistance was closely associated with the plugging of PMNs in the membrane pores. At high pressure differences (12.8 - 10 cmH2O, 7.8 - 5 cmH2O), the relative resistance increased up to finite values, as the filtered volume increased. The variation in the relative resistance was greatly dependent upon the pressure difference or the flow condition. The amount of filtered cell fraction increased with an increase of additional pressure, indicating that the relative resistance was changed according to the rate of PMN plugging and dislodging in the pores of the membrane.

Keywords: Filtration, Leukocyte plugging, Nuclepore membrane, Polymorphonuclear leukocyte, Relative resistance

DOI: 10.3233/BIR-1989-26603

Journal: Biorheology, vol. 26, no. 6, pp. 989-1002, 1989