Filtration pressure and red blood cell deformability: Evaluation of a new device: erythrometer

Issue title: New Methods in Biorheology. Proceedings of the International Symposium held in Nancy, France, 17–18 August 1983. A Satellite Symposium of the Fifth International Congress of Biorheology

Guest editors: J.F. Stoltz and E. Puchelle

Article type: Research Article

Authors: Guéguen, M.^a | Bidet, J.M.^b | Durand, F.^a | Driss, F.^c | Joffre, A.^b | Genetet, B.^a

Affiliations: [a] Centre de Transfusion Sanguine, Rennes | [b] Centre de Transfusion Sanguine, Angers | [c] Hôpital Ste Perrine, Paris

Abstract: The authors have tested a new device that records the filtration pressure of a suspension of red blood cells (R.B.C) passing through a 3μm pore diameter filter. As the flow rate is constant, the pressure increase allows to appreciate an index of the R.B.C deformability. In order to test the performances of this new equipment, the authors have studied the influence of the variation of the following parameters. -Haematocrit from 0.5 to 2%, Buffer composition; time elapsing from drawing to measurement: from hour 0 to hour 3. This analysis has been performed on donors; on packed red cells preserved in CPD, from D0 to D21 and on patients specimens and the results have been compared to the whole blood filtration according to the Reid and Dormandy method. The following parameters were simultaneously measured:-Whole blood viscosity by means of a Low Shear 30 viscosimeter, Intraerythrocyte ATP by bioluminescence, Membrane lipid composition (cholesterol, phospholipids), Scanning electron microscopy. This new apparatus, measuring the filtration pressure seems to be a more insensitive and reproductible method allowing to approach the RBC deformability, and quite useful in clinical haemorheology.

Keywords: Erythrocyte filterability, erythrometer, filtration pressure

DOI: 10.3233/BIR-1984-23S145

Journal: Biorheology, vol. 23, no. s1, pp. 261-265, 1984