Affiliations: INSERM U 284 and Centre Regional de Transfusion Sanguine, Brabois, C.O. n°11, 54511 Vandoeuvre Les Nancy Cedex
Note: [] Accepted by: Editor A.L. Copley
Abstract: In order to define various aspects of platelet rheological activation, samples of whole blood and platelet-rich plasma (PRP) from the same donors were subjected for 5 min to shear rates increasing from 10 to 10000 sec−1 (shear stresses from 10−2 to 30 Pa approximatively) in a Couette type viscometer. The following parameters were measured: erythrocyte hemolysis; lactic dehydrogenase activity; plasma B-Thromboglobulin(B-TG); adenine nucleotides, and platelet photometric aggregation. The experimental results reveal that: In whole blood, hemolysis only reached at maximum 2% of the total hemolysis. Plasma LDH activity increased regularly beyond 500 sec−1, in close correlation with B-TG plasma concentration. In contrast, ADP and ATP levels remained stable up to 1000 sec−1 then increased slowly. In PRP, the LDH, ADP and ATP levels remain practically stable up to shear rates around 5000 sec−1. In contrast, B-TG appeared to be released in plasma at shear rate values of 3000 sec−1 and its progression is only correlated with the other parameters, when the platelet lysis occurred. Finally, a rapid and complete inhibition of platelet aggregation to ADP was observed from 5000 sec−1.
Keywords: Rheology, red blood cell, platelet, shear activation
DOI: 10.3233/BIR-1985-22506
Journal: Biorheology, vol. 22, no. 5, pp. 425-435, 1985
