Affiliations:
Department of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Hungary
Correspondence:
[**]
Address for correspondence: Norbert Nemeth MD, PhD, DSc, Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Nagyerdei krt. 98., Hungary. Tel.: +36-52-416-915; Fax: +36-52-416-915; E-mail: nemeth@med.unideb.hu.
Note: [*] Viktoria Somogyi and Katalin Peto contributed equally to the paper.
Abstract: Background:Age- and gender-related alterations of hemorheological parameters have not been completely elucidated to date. Experiments on older animals may give valuable information on this issue. However, the majority of rheological studies have been performed in young rodents. Objective:We aimed to investigate the influence of aging and gender on hemorheological parameters in rats. Methods:
Coeval male (
n=10
) and female (
n=10
) Wistar (Crl:WI) rats were followed-up over 15 months. Blood samples were obtained from the lateral tail vein at 3, 4, 5, 9, 12, 15 and 18 months of age. Hematological parameters, red blood cell deformability (elongation under shear), osmotic gradient deformability and erythrocyte aggregation were tested. Body weight and the estrus cycle (in females) were also examined.
Results:Erythrocyte aggregation showed age- and gender-related variations. Red blood cell deformability was greater in females and gradually decreased over the 15-month period in both genders. Erythrocyte aggregation was greater in male rats at most ages, but did not show consistent changes with age. Conclusions:The micro-rheological parameters showed age-related alterations with gender differences. The effect of the estrous cycle cannot be excluded in female rats. The results provide reference data for studies of aging in rats and of the mechanism related to age and gender differences in hemorheology.
Keywords: Hemorheology, red blood cell deformability, red blood cell aggregation, gender differences, aging, rat
DOI: 10.3233/BIR-17148
Journal: Biorheology, vol. 54, no. 5-6, pp. 127-140, 2018
