Affiliations: [a] School of Physics and Astronomy, University of St Andrews, UK | [b] Institut de physique de Rennes, Université Rennes 1, CNRS, France
Correspondence:
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Address for correspondence: Institut de physique de Rennes, UMR 6251 Université Rennes 1, CNRS, 263 av. Général Leclerc, F-35042 Rennes, France. E-mail: rdsv.matthieu@gmail.com.
Abstract: Background:Biological cells exhibit complex mechanical properties which determine their responses to applied force. Objective:We developed an optical method to probe the temporal evolution of power-law rheology of single cells. Methods:The method consisted in applying optically a constant mechanical torque to a birefringent microparticle bound to the cell membrane, and observing dynamics of the particle’s in-plane rotation. Results:The deformation dynamics of the membrane followed a power law of time, which directly relates to cytoskeletal prestress as reported in the literature. The temporal evolution of this rheological behaviour, over time scales of several minutes, showed strong variations of the exponent on single adherent cells not subject to any specific treatment. Conclusions:The consistent observation of variations in the exponent suggests that, in their normal activity, living cells modulate their prestress by up to three orders of magnitude within minutes.
