Monomeric green fluorescent protein as a protein standard for small angle scattering

Myatt, Daniel P.; Hatter, Louise; Rogers, Sarah E.; Terry, Ann E.; Clifton, Luke A.

doi:10.3233/BSI-170167

Monomeric green fluorescent protein as a protein standard for small angle scattering

Article type: Research Article

Authors: Myatt, Daniel P.^{; *} | Hatter, Louise | Rogers, Sarah E. | Terry, Ann E. | Clifton, Luke A.

Affiliations: ISIS Spallation Neutron Source, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0QX, UK

Correspondence: [*] Corresponding author. E-mail: daniel.myatt@gmail.com.

Keywords: Green fluorescent protein, small angle scattering, protein standard, biophysics, small angle neutron scattering, small angle X-ray scattering

DOI: 10.3233/BSI-170167

Journal: Biomedical Spectroscopy and Imaging, vol. 6, no. 3-4, pp. 123-134, 2017

Published: 27 December 2017

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Supplementary Materials:

Supplementary data

Abstract

Protein small angle scattering (SAS) has become increasing important in structural biochemistry, due to the increased performance and specification of new instruments and advances in the software and hardware used to analyse the data. Whilst all of this is encouraging, there is a lack of standardised experimental methodology within the community. Although a number of protein standards are currently used in SAS experiments to allow accurate molecular weight determination, each has specific advantages and disadvantages. We therefore propose the use of a mutated monomeric enhanced green fluorescent protein, as a protein standard, abbreviated to m-eGFP. It has a number of advantages over the currently used protein standards, for example it is cheap and easy to produce. It can be expressed in large amounts (>40mg/L) in both hydrogenated and deuterated form. The mutation means it is highly monodisperse and GFP being a beta-barrel structure is thermodynamically stable over a number of days, giving highly reproducible results. We therefore believe m-eGFP is a good protein standard for small angle scattering (SAS).

Abbreviations

σ	1 standard deviation
Å	angstrom
AUC	analytical ultra-centrifugation
BSA	bovine serum albumin
D₂O	deuterium oxide
ddH₂O	double distilled water
eGFP	enhanced GFP
GFP	green fluorescent protein
HSA	human serum albumin
I(0)	intensity at zero angle
kDa	kilodaltons
K_d	binding affinity
m-eGFP	monomeric eGFP
MS	mass spectrometry
M_w	molecular weight
P(r)	distance distribution function
Rg	radius of gyration
S	S(vedberg) unit of sedimentation-coefficient
SAS	small angle scattering
SANS	small angle neutron scattering
SAXS	small angle X-ray scattering
SDS-PAGE	sodium dodecyl sulphate polyacrylamide gel electrophoresis

1.Introduction

Small angle scattering (SAS) using either X-rays or neutrons is a powerful technique [1,2], which requires accurate standard calibration to allow the determination of the molecular weight (M_w) and volume of biomolecules, most commonly proteins [3,4]. Proteins can either be calibrated by comparison to a known protein standard [5,6] or by placing on an absolute scale versus water [7]. The calibration of proteins can be described using the following equation:

(1)Iprot(0)=Istan(0)Cprot×MprotCstan×Mstan

Where, I(0)_prot and I(0)_stan (arbitrary units) are the scattering intensities at zero angle of the protein of interest and the protein standard, c_prot and c_stan are the concentrations (in (M)oles), of the protein of interest and the protein standard, and M_prot and M_stan are the molecular weights (M_w’s) of the protein of interest and the protein standard, respectively (in kilodaltons (kDa)). A number of proteins have been used as calibration standards, the four main ones being: i) lysozyme [6], ii) bovine (BSA) [6,8] or human serum albumin (HSA) [9] and iii) glucose isomerase [5]. For proteins to be considered as a calibration standard they need a number of attributes, specifically being; i) cheap and abundantly available, ii) monodisperse and iii) thermodynamically stable over time, so giving reproducible and expected results, most commonly using Guinier analysis to determine results such as radius of gyration (Rg) and intensity at zero angle I(0) [4,10]. The two classically used protein standards are lysozyme and BSA [6,8]. Both proteins have specific problems. For example, lysozyme has a small molecular weight of only 14.3 kDa, making it a weak scatterer at routine small angle scattering concentrations [6]. BSA and also HSA have aggregation problems due to the oxidation of disulphide bond linkages over time causing oligomerisation [11] and are also sensitive to aggregation by ionizing radiation [12]. More recently, Kozak et al. [5] suggested glucose isomerase from Streptomyces rubiginosus as a potential standard. It has good stability properties overtime and to radiation. Although, as a tetramer of 173 kDa it is quite large compared to many proteins of interest [13]. We therefore propose the use of monomeric enhanced green fluorescent protein (m-eGFP). GFP is a 26.9 kDa monomeric 11-strand beta barrel and like many beta barrels it is extremely thermodynamically stable [14–17]. Enhanced GFP (eGFP) has been mutated to further increase its quantum yield and fluorescence extinction co-efficiency (ε) over standard GFP [18,19]. Standard eGFP is known to homo-dimerise with a binding affinity of 100 μM [17,20]. A binding affinity at this level is problematic for protein concentrations commonly used in small angle scattering (i.e. 1–10 mg/mL), as it results in significant populations of monomers and dimers. This makes easy analysis as a protein standard far from ideal. Fortunately e-GFP can be mutated at a specific amino acid from an alanine to a lysine (A206K) on its dimerization interface [17,21]. This mutation is known to disrupt the dimerization interface, reducing the dimerization binding affinity (K_d) 740-fold to 74 mM [17,21,22]. At a binding affinity of this level, with the protein concentration commonly used in SAS (1–10 mg/mL) over 99% of the protein is monomeric. Utilising SAXS, SANS, mass spectrometry (MS) and analytical ultracentrifugation (AUC) experiments alongside SDS-PAGE gels, we show that m-eGFP is a particularly good small angle scattering protein standard. It is easy to express in large amounts in hydrogenous and deuterated media and is therefore cheap to produce. M-eGFP is also highly florescent and amiable to freeze-dried storage and weighing out, which allows for two checks for protein concentration determination, so reducing concentration errors, which have been observed to be significant in other protein concentration assays [23,24].

2.Materials and methods

2.1.Molecular biology and protein expression

The eGFP plasmid (QBio-GENE – http://www.qbiogene.com/technical/maps/txt/s-pQBI-T7-BFP.txt plus S66T and H67Y mutations) was provided as a gift from Prof. Cameron Neylon (Curtin University, Australia). Mutagenesis of eGFP was performed using an Agilant QuikChange II Site-Directed Mutagenesis kit and the custom sense and antisense primers designed using the Agilant QuikChange Primer Design site (http://www.genomics.agilent.com/primerDesignProgram.jsp). m-eGFP was produced by a single amino acid mutation at amino acid position 206 (A206K), specifically an alanine (A-GCC) being mutated to a lysine (K-AAA). To confirm mutagenesis sequencing was undertaken by Source Bioscience Ltd. (Oxford, UK) using the purified plasmid DNA and a customised forward sequencing primer of GGTGATGCAACATACGGAAA starting at DNA sequence number 4743, to allow sequencing of the mutation at sequence number 5256. The plasmid was transformed either into BL21(DE3) E. coli cells and grown in LB media to produce hydrogenous protein or Slantes buffer OD-5 E. coli ²H media (Silantes GmbH, Germany) for deuterated protein in a BioFlo 110 Bioreactor (New Brunswick, UK) to an OD₆₀₀ of 1.2, then induced with 0.5 mM IPTG for 4 hrs at 37°C. The cells were then harvested at 3000 rpm in F8S (Thermo Sorvell™) centrifuge tubes. The cells were lysed using Bugbuster™ (Novagen, UK) and 5 minutes ON/OFF 10 second cycles of sonication. The lysate was passed through a Colbolt IMAC Hitrap™ column (General Electric Healthcare, UK) and the protein was eluted by increasing the imidazole concentration to 200 mM in a Tris pH 7.5, 150 mM NaCl buffer. After purification the 5-His tag was enzymatically cleaved using the Sortase A enzyme at the LPXTG motif (see Supplementary Material Fig. 1) and the protein was then dialysed into a 20 mM Tris, 150 mM NaCl, pH 7.5 buffer in ddH₂O before experimentation.

2.2.SDS-PAGE gels

A 10 μL sample of 10 μM eGFP and m-eGFP was run alongside a Sigma Wide range marker (Sigma Aldrich, UK) at 150V on a 12% SDS PAGE Tris-gels using a Bio-Rad Mini Protean (Biorad, UK) electrophoresis at 298K. The gel was stained in Biosafe™ Coomassie blue (Biorad, UK).

2.3.Mass spectrometry

Mass spectrometry was performed on a Micromass Q-Tof Micro Mass spectrometer (Waters, UK) on a 10 μM hydrogenous m-eGFP sample in 20 mM phosphate and 150 mM NaCl at pH 7.5 in ddH₂O.

2.4.UV absorption

The concentration of m-eGFP was determined by its A₂₈₀ absorption value. Samples were measured using an Eppendorf microvolume cuvette on a GeneQuant 1300 (General Electric Healthcare, UK) spectrophotometer. M-eGFP concentrations were determined using a calculated extinction co-efficient of 22,000M−1cm−1 at A₂₈₀ [16,17].

2.5.Analytical ultracentrifugation

Sedimentation velocity experiments were conducted at 20 °C and 129,024 g (RCF) in a Beckman XL-I analytical ultracentrifuge and Rayleigh interference data was recorded for 999 scans at 1 minute intervals. The resulting concentration distributions were processed exactly the same for each spectra and analysed by the SEDFIT program [25] to obtain c(s) – s distributions for hydrogenous eGFP and m-e GFP at 1, 5 and 10 mg/mL in a 20 mM Phosphate, 150 mM NaCl buffer at pH 7.5 in ddH₂0 conducted at 20 °C. The experimental hydrodynamic parameters were compared to theoretical parameters calculated using the GFP crystal structure (Pdb: 1EMA) [14] in the HYDROPRO software program [26].

2.6.SAXS data collection

SAXS measurements of a hydrogenous m-eGFP protein in hydrogenous buffer were performed at three different concentrations (1, 5 and 10 mg/mL) on the B21 beamline at the Diamond Light Source Ltd. [27] (UK) using the automated BIOSAXS robot for sample loading at 15 °C. B21 was operated at a fixed camera length of 3.9 m and an energy of 12.4 keV to collect data between 0.015 and 0.3Å−1. The data was collected on a 2M Pilatus detector (Dectris, UK). The data from the two dimensional area detector was converted into one-dimensional intensity profiles by radial averaging. The data was then corrected to allow for sample transmission and background scattering (using 20 mM Tris, 150 mM NaCl at pH 7.5 in ddH₂O as a reference). The data were processed using the DAWN software package (Diamond Light Source Ltd., UK) [28].

2.7.SANS data collection

SANS experiments of hydrogenous m-eGFP protein in deuterated buffer and deuterated protein in hydrogenous buffer at three different concentrations (1, 5 and 10 mg/mL) were conducted at the ISIS Spallation Neutron Source (UK) using the LOQ and SANS2d Instruments [29,30]. LOQ is a time of flight (TOF) SANS instrument with a two-dimensional 64 cm by 64 cm 3He–CH₄ ORDELA detector with 5 mm resolution. Neutron wavelengths of between 2.2 and 10 Å were utilised and a distance of 4.1 m between sample position and the detector was used to measure scattering profiles. SANS2d is also a time of flight (TOF) instrument with two two-dimensional 96.5 cm by 96.5 cm 3He–CF₄ filled ORDELA detectors with a 5 mm resolution. Neutron wavelength between 2 and 14 Å were utilised and a distance of between 2 and 12 m between sample position and detector were used to measure the scattering profile. Both instruments used absolute intensities for scattering determined using a partially deuterated polymer standard. All measurements were carried out at room temperature in sealed 1 mm pathlength quartz cuvettes (Hellma Analytics) during data collection. The data from the two dimensional area detector was converted into one-dimensional intensity profiles by radial averaging. The SANS data was then corrected to allow for sample transmission and background scattering (using either 20 mM Tris, 150 mM NaCl at pH/pD 7.5 in ddH₂O for deuterated protein samples or D₂O for hydrogenous protein samples as a reference).The data was processed using the Mantid software package (ISIS Neutron and Muon Source, UK) [31].

2.8.SAS data analysis

For SANS measurements, theoretical estimates of the scattering length density of the protein and estimates of its intensity at zero angle I(0) were performed using the Biological Scattering Length Density Calculator (http://psldc.isis.rl.ac.uk/Psldc/). For all SAS measurements, Guinier analysis was performed using the ISIS Biological scattering Guinier webtool (http://psldc.isis.rl.ac.uk/Guinier). Data was taken in line with globular proteins from a minimum q×Rg=0.5 to a maximum q×Rg=1.3. Distance distribution functions (P(r)) were calculated using GNOM [32–35]. Both methods allow the determination of the radius of gyration (Rg) and the zero angle scattering intensity (I(0)) of the sample. Distance distribution functions (P(r)) also provide information on other parameters; such as the maximum diameter (D_max) and overall shape of the particle in the sample [4,36]. The experimental data was compared to the available X-ray crystal structure of monomeric green fluorescent protein Pdb: 1EMA, using CRYSOL [37].

Fig. 1.

A 12% SDS-PAGE of a eGFP (middle) and m-eGFP (right) run alongside a Sigma Wide Range marker (left).

Fig. 2.

A mass spectrum of a hydrogenous m-eGFP protein sample in hydrogenous buffer. Protein buffer was 20 mM phosphate, 150 mM NaCl at pH 7.5.

Fig. 3.

A sedimentation velocity analytical ultracentrifugation experiment using Rayleigh interference optics of hydrogenous m-eGFP at 1 (black – solid line), 5 (red – dash line) and 10 mg/mL (green – dot line) concentrations. The sedimentation coefficient distribution was obtained using SEDFIT analysis [25]. The sample was run in a 20 mM phosphate, 150 mM NaCl buffer in ddH₂O conducted at 20 °C and 129,024 g (RCF).

Fig. 4.

The crystal structure of Green Fluorescent Protein (GFP) monomer – Pdb: 1EMA [14].

3.Results and discussion

The SDS-PAGE gel (see Fig. 1) shows a single band just below the 29 kDa marker as expected from the calculated molecular weight of full-length hydrogenous m-eGFP (28.6 kDa – see Supplementary Material Fig. 1 – for the full coding sequence). No other significant protein bands were observed on the SDS-PAGE gel, indicative of a highly purified sample. The mass spectrum (see Fig. 2) of the same sample shows one single peak with a molecular weight of 28.6 kDa, corresponding to the hydrogenous m-eGFP. As a final analysis of sample purity and homogeneity, three concentrations of m-eGFP (1, 5 and 10 mg/ml) were run in an analytical ultracentrifuge in a sedimentation velocity experiment using Rayleigh interference optics (see Fig. 3). As expected the m-eGFP showed only one peak at 1.9 S(vedberg) at each of the three concentrations, this is again indicative of a monodisperse, high purity sample. The standard, non-mutated, eGFP sample (Supplementary Material – Fig. 2) was also ran at the same three concentrations (1, 5 and 10 mg/ml) and showed two distinct populations of species in slow exchange [38]. The two species were confirmed as being the monomer at 1.9 S and the dimer at 2.5 S, by calculating theoretical Svedberg values for the monomer and dimer of eGFP using the HYDROPRO software [26] and the crystal structures of both proteins (Pdb: 1EMA [14] and 1GFL [15], respectively – see Fig. 4 and Supplementary Material Fig. 3) As expected the dimer increased as a percentage of the total concomitant to increasing protein concentration in accordance with a binding affinity of 100 μM (see Supplementary Fig. 1) [17]. The dimer made up 39, 60 and 70% of the total species at 1, 5 and 10 mg/mL respectively (See Supplementary Material Equation 1 and Table 1 for the full derivation of monomer: dimer binding and the theoretical population percentages versus AUC experimental results). After mutation of e-GFP, to monomeric eGFP (m-eGFP), disrupting its dimersation interface, the dimersation binding affinity increases 740 fold to 74 mM [17], this means the monomer is over 99% of m-eGFP at the experimental concentrations (see Supplementary Material Table 1).

Fig. 5.

Small angle X-ray scattering (SAXS) curves of three different concentrations of hydrogenous m-GFP in hydrogenous buffer, at 1 mg/mL (green – flat line), 5 mg/mL (red – diagonal cross) and 10 mg/mL (black – cross line) concentrations. The spectra is shown in Log I(q) versus Logq. Data points are plotted where the error bars are less than 5% of the data point value. The protein is in 20 mM Phosphate, 150 mM NaCl buffer at pH 7.5 in ddH₂O. The inset show the corresponding Guinier plots of the SAXS scattering curves, with the calculated radius of gyration (Rg) and intensity at zero angle (I(0)).

Fig. 6.

Small angle neutron scattering (SANS) curves of three different concentrations of deuterated m-GFP in hydrogenous buffer, at 1 mg/mL (green – diamond), 5 mg/mL (red – square) and 10 mg/mL (black – ellipse) concentrations. The spectra is shown in LogI(q) versus Logq. Data points are plotted where the error bars are less than the value of the data point. The protein is in 20 mM Phosphate, 150 mM NaCl buffer in ddH₂O. The inset shows a Guinier plots of m-eGFP for the SANS curves with the calculated radius of gyration (Rg) and intensity at zero angle (I(0)).

Three concentrations of m-eGFP were then run in the SAXS and SANS experiment to determine the intensity at zero angle I(0) and radius of gyration (Rg) of m-eGFP (for the results see in Figs 5 and 6 and Supplementary Material Table 2). From the three concentrations in both SAXS and SANS we get consistent radius of gyrations (Rg) within a 95% confidence level. For SAXS (see Fig. 5), hydrogenous protein samples in hydrogenous buffer, gave radius of gyrations (Rg’s) of 19.48, 20.29 and 20.49 Å (to 2 d.p) for 1, 5 and 10 mg/ml concentrations, respectively. Whilst for SANS (see Fig. 5), deuterated protein samples in hydrogenous buffer, gave radius of gyrations (Rg’s) of 17.17, 19.59 and 20.80 Å (to 2 d.p) for 1, 5 and 10 mg/mL, repectively. Both the techniques, as expected, are in good agreement, with the 1 mg/mL value for SAXS and particularly SANS values showing the largest standard deviation away from the mean. The increased noise in SAXS and SANS data at 1 mg/ml was due to a lower concentration. All of the radius of gyration values are also in good agreement with the CRYSOL [37] model value of 16.98 Å for the smaller crystal GFP structure (Pdb: 1EMA) without the N- and C-terminal tails [14]. Intensity at zero angle I(0) values were also calculated for both techniques. These values are dependant on a number of parameters including the sample concentration and buffer content (i.e D₂O percentage for SANS). As expected, the values increased concomitantly with concentration, both in SAXS and SANS. For SAXS (see Fig. 5), a hydrogenous protein sample in hydrogenous buffer gave zero angle intensity I(0) values of 0.04, 0.20 and 0.43cm−1 (to 2 d.p) for 1, 5 and 10 mg/mL respectively. Whilst for SANS (see Fig. 5), a deuterated sample in hydrogenous buffer (used to increase the scattering signal by increasing contrast variation) gave zero angle intensity I(0) values of 0.06, 0.34 and 0.72cm−1 (to 2 d.p) for 1, 5 and 10 mg/mL respectively. Again, both sets of values are within the 95% confidence level of standard deviation. The SANS values are in good agreement with rough estimated values of 0.10, 0.48 and 0.96cm−1 (to 2 d.p) for zero angle intensity I(0), for a globular protein made using the Biological Scattering Calculator [39] for a 90% deuterated m-eGFP protein, with 90% hydrogen exchange in 100% ddH₂O buffer.

Fig. 7.

The distance distribution plot (P(r)−r) of m-eGFP taken using the SAXS (black – cross), SANS (red – diagonal cross) and the 1EMA theoretical crystal structure (green – flat line) distance distribution. Distance distribution performed using the 10 mg/mL m-eGFP data shown in Figs 5 and 6 and theoretical crystal structure (Pdb: 1EMA) calculated using Crysol [37].

A distance distribution function (P(r)) analysis using the 10 mg/mL SAXS and SANS m-eGFP data was then performed (see Fig. 7). Distance distribution provides a number of complimentary parameters to the Guinier analysis. As well as radius of gyration (Rg) and intensity at zero angle I(0), other important values such as the particles maximum diameter (D_max) and particle shape are also determined [4]. The distance distribution analysis (P(r)) is indicative of a globular protein of length ∼50Å, as shown in the theoretical GFP crystal structure (see Fig. 7 – green line). The increased tail observed in the experimental SAXS and SANS data (see Fig. 7 – black and red lines, respectively) is from the N- and C-terminals of the particles, not present in the GFP crystal structure data [14] (Fig. 7 – green line). The experimental values obtained (Fig. 7 – black and red line) are therefore in good agreement with the theoretical data calculated from the protein data bank structure (Pdb: 1EMA [14] – Fig. 7 – green line).

A key factor in developing a new biological small angle scattering standard is the particles stability over time. To test m-eGFP’s stability, SANS data was taken for a hydrogenous m-eGFP protein sample in deuterated buffer over 1, 15 and 30 days at three concentrations (1, 5 and 10 mg/mL). A Guinier analysis of the data was then performed to determine any changes in the zero angle intensity I(0) and radius of gyration (R_g) at each concentration and time point. The results of this analysis (see Fig. 8 and Supplementary Material Table 3) give the average radius of gyration (Rg) and intensity at zero angle (I(0)) value of the 9 spectra (3 different time points (1, 15 and 30 days) at 3 concentrations (1, 5 and 10 mg/mL)). The average radius of gyration (Rg) 18.14Å±(1σ) 0.82 (to 2 d.p), and intensity at zero angle (I(0)) value of 0.41cm−1±(1σ) 0.03 (to 2 d.p) are obtained (see Supplementary Material Table 3 full the results). All of the Guinier data points (see Fig. 8) are within the standard 95% confidence level (1.96 σ). The results therefore show no significant deviation from the average values obtained. The results with the largest standard deviation are, as expected, the 1 mg/mL concentration spectra. This is due to the increased error observed in the spectra from the decreased concentration used. This is particularly apparent for time of flight instruments such as Loq and Sans2d as they have less neutron flux in the Guinier region (ISIS Neutron and Muon Source, UK) [29,30]. There is a small increase in radius of gyration with concentration, this is likely due to the decrease of error in the Guinier region at higher concentrations, although very small amounts of concentration dependent dimerisation/aggregation can not be fully discounted over such a long time period. Also, the intensity at zero angle I(0) increases concomitantly with concentration as expected. The results (see Fig. 8 and Supplementary Material Table 3) show m-eGFP is not susceptible to the dimerisation and aggregation problems observed in other small angle scattering protein standards, most notable BSA [11 ,12].

Fig. 8.

A time-course at 1, 15 and 30 days of a sample of hydrogenous m-eGFP in D₂O buffer, showing the radius of gyration (Rg) (top) and intensity at zero angle (I(0)) (bottom) of the three different concentrations of m-eGFP (1, 5 and 10 mg/mL). The cross-points of 1, 5 and 10 mg/mL are denoted by a black ellipse, red square and green diamond, whilst the mean average of the three concentrations is denoted by a blue triangle. The error bars denote the 95% confidence interval (1.96 σ – standard deviation of a standard statistical distribution).

A time-course at 1, 15 and 30 days of a sample of hydrogenous m-eGFP in D2O buffer, showing the radius of gyration (Rg) (top) and intensity at zero angle (I(0)) (bottom) of the three different concentrations of m-eGFP (1, 5 and 10 mg/mL). The cross-points of 1, 5 and 10 mg/mL are denoted by a black ellipse, red square and green diamond, whilst the mean average of the three concentrations is denoted by a blue triangle. The error bars denote the 95% confidence interval (1.96 σ – standard deviation of a standard statistical distribution).

4.Conclusions

After the biophysical characterisation shown here, we believe that m-eGFP has all the key characteristics required for use as a protein standard in small angle scattering experiments. Specifically it is easy to obtain, expresses in large amounts in hydrogenous and deuterated media, is highly monodisperse, has high stability over time and also hands storage well. M-eGFP’s fluorescent nature also allows for easy concentration determination. We believe the development of m-eGFP as a SAS protein standard is timely, with SAS data becoming increasingly important in structural biology and recent calls for more standardisation of biological SAS data [40–42].

Acknowledgements

The authors wish to thank Prof. Cameron Neylon for providing the e-GFP plasmid (Curtin University), Dr Joanne Nettleship (OPPF, Research Complex at Harwell) for running the mass spectrometer sample and Dr Richard Heenan (ISIS Neutron and Muon Source, UK) for discussion on the calculation of theoretical protein zero angle intensity values for biomolecules. The m-eGFP plasmid construct is available upon request to ISIS Neutron and Muon Source (Oxford, UK).

Declarations of interest

The authors declare that they have are no competing interests with the manuscript.

Funding information

Funding was provided in-house for the relevant consumables and beamtime to DPM as Biological Research Scientist at ISIS Neutron and Muon Source (Oxford, UK).

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