Structural response of genomic DNA from grapevine (Vitis vinifera L.) varieties to microwaves irradiation: A Fourier transform infrared spectroscopy assessment

Article type: Research Article

Authors: Tripon, Carmen^a | Muntean, Cristina M.^{a; *} | Surducan, Emanoil^a | Bratu, Ioan^a | Halmagyi, Adela^b | Coste, Ana^b

Affiliations: [a] National Institute for Research & Development of Isotopic and Molecular Technologies, 67-103 Donat Str., 400293 Cluj-Napoca, Romania | [b] Institute of Biological Research branch of National Institute of Research and Development for Biological Sciences, 48 Republicii Str., 400015 Cluj-Napoca, Romania

Correspondence: [*] Corresponding author: Dr. Cristina M. Muntean. E-mail: cmuntean@itim-cj.ro.

Abstract: In this work, Fourier transform infrared (FT-IR) spectra of untreated and microwaves irradiated genomic DNAs extracted from leaves of different grapevine varieties, respectively, have been analyzed between 1800–800 cm−1, in order to investigate their screening characteristic features and their structural response to microwaves treatment at 869–894 MHz frequency band. FT-IR vibrational modes for each of these cases, spectroscopic band assignments and structural interpretations of genomic DNAs are reported. No significant microwaves damage, concerning DNA bases structure, base pairing and base unstacking was found. Conformational changes of specific DNA segments and effects on deoxyribose seem to appear after microwaves irradiation. Grapevine varieties dependent microwaves influence on the structure of DNA has been found. Best nucleic acids structural tolerance upon microwave irradiation, as compared with the non-exposed DNA has been found in the case of genomic DNA isolated from ‘Chasselas Doré’ grapevine variety.

Keywords: Genomic DNA, grapevine varieties, FT-IR spectroscopy, microwaves irradiation

DOI: 10.3233/BSI-160138

Journal: Biomedical Spectroscopy and Imaging, vol. 5, no. 3, pp. 295-312, 2016